HDAC5 抗体 (pSer661)
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- 抗原 See all HDAC5 抗体
- HDAC5 (Histone Deacetylase 5 (HDAC5))
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抗原表位
- pSer661
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适用
- 人, 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This HDAC5 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Dot Blot (DB), Fluorescence Microscopy (FM)
- 原理
- HDAC5 (internal) Antibody
- 交叉反应 (详细)
- This antibody is specific towards HDAC5.
- 产品特性
- Synonyms: rabbit anti-HDAC-5 antibody, HDAC5, HDAC 5, HD 5 antibody, histone deacetylase 5 antibody, Histone deacetylase-5, HD5 antibody, NY-CO-9
- 纯化方法
- Anti-HDAC5 was affinity purified from monospecific antiserum by immunoaffinity chromatography.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: HDAC5 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the internal region surrounding phosphoserine 661 of human HDAC5.
Immunogen Type: Conjugated Peptide
- Top Product
- Discover our top product HDAC5 Primary Antibody
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- 应用备注
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Immunohistochemistry Dilution: 1:100-1:500
Application Note: Anti-HDAC5 antibody has been tested in ELISA, dot blot, and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~124 kDa corresponding to the appropriate cell lysate or extract.
Western Blot Dilution: 0.2 μg/mL
ELISA Dilution: 1:20,000 - 1:60,000
IF Microscopy Dilution: 1:100-1:500
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.12 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 50 % (v/v) Glycerol
Preservative: None - 储存液
- Without preservative
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 6 months
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- 抗原
- HDAC5 (Histone Deacetylase 5 (HDAC5))
- 别名
- HDAC5 (HDAC5 产品)
- 背景
- Background: HDAC5 is a member of the class II mammalian histone deacetylase family, which is structurally related to yeast HDA1. Human HDAC5 is composed of 1122 amino acid residues. The deacetylase domain of HDAC5 is located at the C-terminal half of the molecule. The N-terminal non-deacetylase domain does not show any significant homology with any published sequence. Both domains are required for HDAC5-mediated repression of gene transcription. HDAC5 interacts with a growing number of transcriptional factors including MEF2A as well as other HDAC proteins. The interacting complexes bind to specific regions of chromatin and regulate gene transcription in these regions. Anti-HDAC5 antibodies are ideal for researchers interested in Breast Cancer, Cancer, Cell Cycle and Replication, Chromatin Research, Epigenetics, and Histone Deacetylases research.
- 基因ID
- 10014
- NCBI登录号
- NP_005465
- UniProt
- Q9UQL6
- 途径
- Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Monocarboxylic Acid Catabolic Process
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