Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Fluorescence Microscopy (FM)
交叉反应 (详细)
Cross reactivity with HDAC5 from other sources has not been determined.
纯度
Anti-HDAC5 was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific towards HDAC5. A BLAST analysis was used to suggest cross-reactivity with Human based on 100 % sequence homology.
内毒素水平
Low Endotoxin : No
免疫原
HDAC5 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the internal region of human HDAC5. Immunogen Type: Peptide
Anti-HDAC5 antibody is useful for ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~124 kDa corresponding to the appropriate cell lysate or extract. ELISA Dilution: 1:20.000 - 1:60.000 Immunohistochemistry Dilution: 1:100-1:500 IF Microscopy Dilution: 1:100-1:500 Western Blot Dilution: 0.2 µg/mL
限制
仅限研究用
状态
Liquid
浓度
1.12 mg/mL
缓冲液
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Stabilizer: 50 % (v/v) Glycerol
储存条件
4 °C/-20 °C
储存方法
Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.
有效期
Expiration date is six (6) months from date of opening.
HDAC5 is a member of the class II mammalian histone deacetylase family, which is structurally related to yeast HDA1. Human HDAC5 is composed of 1122 amino acid residues. The deacetylase domain of HDAC5 is located at the C-terminal half of the molecule. The N-terminal non-deacetylase domain does not show any significant homology with any published sequence.Both domains are required for HDAC5-mediated repression of gene transcription. HDAC5 interacts with a growing number of transcriptional factors including MEF2A as well as other HDAC proteins. The interacting complexes bind to specific regions of chromatin and regulate gene transcription in these regions. Anti-HDAC5 antibodies are ideal for researchers interested in Breast Cancer, Cancer, Cell Cycle and Replication, Chromatin Research, Epigenetics, and Histone Deacetylases research. Synonyms: Antigen NY-CO-9, EC 3.5.1.98, FLJ90614, HD5, histone deacetylase 5, KIAA0600, NY-CO-9