Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Fluorescence Microscopy (FM)
交叉反应 (详细)
Cross reactivity with ARHGAP22 pS22 from other sources has not been determined.
纯度
Anti-ARHGAP22 pS22 was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific for phosphorylated ARHGAP22 at Serine 22. It also recognizes the S397-> A mutation but not the S22-> A mutation. A BLAST analysis was used to suggest cross-reactivity with Mouse, Rat and Human based on 100 % sequence homology.
内毒素水平
Low Endotoxin : No
免疫原
ARHGAP22 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the region surrounding mouse pS22 region of ARHGAP22. Immunogen Type: Peptide
Anti-ARHGAP22 pS22 antibody is useful for ELISA, Western Blot and Immunostaining. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~77.8 kDa corresponding to the appropriate cell lysate or extract. ELISA Dilution: 1:20.000 - 1:60.000 Immunohistochemistry Dilution: 1:100-1:500 IF Microscopy Dilution: 1:100-1:500 Western Blot Dilution: 1 µg/mL
限制
仅限研究用
状态
Liquid
浓度
1.07 mg/mL
缓冲液
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Stabilizer: 50 % (v/v) Glycerol
储存条件
4 °C/-20 °C
储存方法
Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.
有效期
Expiration date is six (6) months from date of opening.
抗原
ARHGAP22
(rho GTPase Activating Protein 22 (ARHGAP22))
ARHGAP22 is a Rho GTPase-activating protein involved in the signal transduction pathway that regulates endothelial cell capillary tube formation during angiogenesis. It acts as a GTPase activator for RAC1 by converting it to an inactive GDP-bound state and also inhibits RAC1-dependent lamellipodia formation. It may also play a role in transcription regulation via its interaction with VEZF1, by regulating activity of the endothelin-1 (EDN1) promoter. Anti-ARHGAP22 [p Ser22] antibody is ideal for researchers interested in Diabetes Research, Lipid and Metabolism research. Synonyms: Rho-type GTPase-activating protein 22, RHOGAP2, RhoGAP2, Rho GTPase activating protein 22, rho GTPase-activating protein 22