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- 抗原 See all CX3CL1 抗体
- CX3CL1 (Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1))
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This CX3CL1 antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), ELISA
- 交叉反应 （详细）
- Cross reactivity with Fractalkine from other sources has not been determined.
- 纯度
- Anti-Fractalkine is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for human Fractalkine protein. A BLAST analysis was used to suggest cross-reactivity with Fractalkine from human sources based on 100 % homology with the immunizing sequence. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin and anti-Rabbit Serum.
- 内毒素水平
- Low Endotoxin : No
- 免疫原
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Anti-Fractalkine antibody was prepared from rabbit antiserum after repeated immunizations with a truncated form of recombinant human Fractalkine protein from E. coli.
Immunogen Type: Recombinant Protein - 亚型
- IgG
- Top Product
- Discover our top product CX3CL1 Primary Antibody
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anti-Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1) (AA 25-332) antibody
CX3CL1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1) (AA 365-397) antibodyCX3CL1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1) (AA 301-397) antibodyCX3CL1 适用: 人, 大鼠, 小鼠 WB, ELISA, FACS, ICC, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1) antibodyCX3CL1 适用: 人 WB, IF 宿主: 兔 Monoclonal unconjugated
anti-Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1) (AA 25-100) antibodyCX3CL1 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1) (Internal Region) antibodyVerified CX3CL1 适用: 人 ELISA, IHC, FACS 宿主: 山羊 Polyclonal unconjugated
anti-Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1) (AA 81-336) antibodyCX3CL1 适用: 人 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1) (N-Term) antibodyCX3CL1 适用: 人, 大鼠, 小鼠, Cow, 犬, 豚鼠, 马, 兔, Saccharomyces cerevisiae, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1) (AA 272-306) antibodyCX3CL1 适用: 人 WB, FACS 宿主: 兔 Polyclonal RB56920 unconjugated
anti-Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1) (AA 83-330) antibodyCX3CL1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Fractalkine purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~42 kDa in size corresponding to the human Fractalkine protein by western blotting in appropriate cell lysate or extract.
ELISA Dilution: 1:20.000 - 1:50.000
Fluorochrome Protein Value: 10-20
Western Blot Dilution: 1:1000-1:2000 - 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Restore with 100 µL deionized water (or equivalent)
- 浓度
- 1 mg/mL
- 缓冲液
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0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free - 储存条件
- 4 °C/-20 °C
- 储存方法
- Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- Expiration date is one (1) year from date of opening.
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- 抗原
- CX3CL1 (Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1))
- 别名
- Fractalkine (CX3CL1 产品)
- 背景
- Fractalkine (CX3CL1) is an atypical chemokine that was the first of a fourth chemokine motif (CX3C). It is thought to function as a T cell and monocyte chemotractant and is produced by non-haemopoietic cells. Fractalkine is made in a soluble and membrane bound form in activated endothelial cells which is thought to promote adhesion of leukocytes. The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils, while the cell-bound chemokine promotes strong adhesion of leukocytes to activated endothelial cells, where it is primarily expressed. It is found predominantly in the small intestine, colon, testis, prostate, heart, brain, lung, skeletal muscle, kidney and pancreas. Fractalkine may play a role in regulating leukocyte adhesion and migration processes at the endothelium by binding to CX3CR1. Recombinant human Fractalkine is a non-glycosylated protein with a molecular weight of 8.6 kDa. Anti-Fractalkine antibody is ideal for researchers interested in cytokines and stem cell research. Synonyms: CX3CL1, Fractalkine, Chemokine (C-X3-C motif) ligand 1, C-X3-C motif chemokine 1, CX3C membrane-anchored chemokine, Neurotactin, Small-inducible cytokine D1, FKN, NTT, SCYD1, A-152E5.2
- 基因ID
- 6376
- NCBI登录号
- NP_002987
- UniProt
- P78423
- 途径
- Synaptic Membrane
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