ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
交叉反应 (详细)
Cross reactivity against Albumin from other tissues and species may occur but has not been specifically determined.
纯化方法
Bovine Serum Albumin antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Albumin (Bovine Serum).
内毒素水平
Low Endotoxin : No
免疫原
Anti-Bovine Serum Albumin antibody was produced by repeated immunizations with bovine serum albumin. Immunogen Type: Native Protein
BSA
适用: Cow, 山羊, 人, 小鼠, 大鼠
WB, IF (cc), IF (p)
宿主: 兔
Polyclonal
AbBy Fluor® 680
应用备注
Anti-ALB antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity. ELISA Dilution: 1:20.000 - 1:100.000 Immunohistochemistry Dilution: 1:1.000 - 1:5.000 Western Blot Dilution: 1:2.000-1:10.000
限制
仅限研究用
状态
Lyophilized
溶解方式
Restore with 100 µL deionized water (or equivalent)
浓度
1 mg/mL
缓冲液
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
储存条件
4 °C/-20 °C
储存方法
Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.
有效期
Expiration date is one (1) year from date of opening.
PRO0883 antibody, PRO0903 antibody, PRO1341 antibody, albumin antibody, ALB antibody
背景
Bovine Serum Albumin antibody detects BSA. Serum albumin is the main protein of plasma. It has a good binding capacity for water, Ca2+, Na+, K+, fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. ALB is the major zinc transporter in plasma, it typically binds about 80 % of all plasma zinc. BSA antibody is ideal for investigators involved in Cell Signaling, Neuroscience and Signal Transduction research. Synonyms: ALB antibody, Albumin antibody, Allergen Bos d 6 antibody, BSA antibody, DKFZp779N1935 antibody, Anti-BSA