LPO 抗体 (Biotin)
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- 抗原 See all LPO 抗体
- LPO (Lactoperoxidase (LPO))
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适用
- Cow
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宿主
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绵羊
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克隆类型
- 多克隆
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标记
- This LPO antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), Immunoprecipitation (IP), ELISA, Dot Blot (DB)
- Supplier Product No.
- 200-606-146-0100
- Supplier
- Rockland
- 原理
- Anti-Lactoperoxidase Antibody Biotin Conjugated
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Sheep Serum as well as purified and partially purified Lactoperoxidase [Bovine Milk].
- 产品特性
- Synonyms: sheep anti-Lactoperoxidase biotin conjugated Antibody, biotin conjugated sheep anti-Lactoperoxidase Antibody, LPO antibody, Salivary peroxidase antibody, SAPX antibody, SPO antibody
- 纯化方法
- Anti-Lactoperoxidase (Bovine Milk) (Sheep) Antibody Biotin Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- 免疫原
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Immunogen: Lactoperoxidase [Bovine Milk]
Immunogen Type: Native Protein
- 亚型
- IgG
- Top Product
- Discover our top product LPO Primary Antibody
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anti-Lactoperoxidase (LPO) (AA 351-589) antibody
LPO 适用: 大鼠 WB, IP, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Lactoperoxidase (LPO) (AA 305-498) antibodyLPO 适用: 小鼠 WB, IP, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Lactoperoxidase (LPO) (AA 244-346) antibodyLPO 适用: 人 WB, IP, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Lactoperoxidase (LPO) (AA 351-589) antibodyLPO 适用: 大鼠 WB, IP, IHC, ICC 宿主: 小鼠 Monoclonal 15# unconjugated
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- 应用备注
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Application Note: Anti-Lactoperoxidase Biotin Conjugated Antibody has been tested by Dot blot and western blot and is suitable to be assayed against 1.0 μg of Lactoperoxidase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:6,000 of the reconstitution concentration is suggested for Anti-Lactoperoxidase.
Western Blot Dilution: 1:50 - 1:500
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:500 - 1:2,000
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 10 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- LPO (Lactoperoxidase (LPO))
- 别名
- Lactoperoxidase (LPO 产品)
- 背景
- Background: Lactoperoxidase antibody recognizes the lactoperoxidase protein. Lactoperoxidase catalyzes the oxidation of a number of inorganic and organic substrates by hydrogen peroxide. Lactoperoxidase plays an important role in killing bacteria in milk. Lactoperoxidase conjugated to Biotin is used to detect the specific target of the lactoperoxidase protein. Lactoperoxidase conjugated to Biotin is suitable for researchers in immunology and biochemistry.
- 基因ID
- 280844
- NCBI登录号
- NP_776358
- UniProt
- P80025
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