Aspartate Transaminase 抗体 (Biotin)
Ast
适用: Pig
ELISA, WB
宿主: 绵羊
Polyclonal
Biotin
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- 抗原 See all Aspartate Transaminase (Ast) products
- Aspartate Transaminase (Ast)
- 适用
- Pig
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宿主
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绵羊
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克隆类型
- 多克隆
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标记
- This Aspartate Transaminase antibody is conjugated to Biotin
- 应用范围
- ELISA, Western Blotting (WB)
- 交叉反应 （详细）
- Cross reactivity against Aspartate Aminotransferase (AST) from other sources may occur but has not been specifically determined.
- 纯度
- Anti-Aspartate Transaminase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Sheep Serum as well as purified and partially purified Aspartate Aminotransferase (AST) [Pig Heart].
- 内毒素水平
- Low Endotoxin : No
- 免疫原
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Aspartate Aminotransferase (AST) [Pig Heart]
Immunogen Type: Native Protein - 亚型
- IgG
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anti-Aspartate Transaminase (Ast) antibody (HRP)
Ast 适用: Pig ELISA, WB 宿主: 绵羊 Polyclonal HRP
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- 应用备注
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Anti-Aspartate Transaminase has been assayed against 1.0 µg of Aspartate Aminotransferase (AST in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4.000 to 1:20.000 of the reconstitution concentration is suggested for this product. This product has been assayed against 1.0 µg of Aspartate Aminotransferase (AST in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4.000 to 1:20.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:25.000
Western Blot Dilution: 1:500 - 1:3.000 - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 10 mg/mL
- 缓冲液
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0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free - 注意事项
- Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
- 有效期
- Expiration date is one (1) year from date of opening.
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- 抗原
- Aspartate Transaminase (Ast)
- 别名
- Aspartate Transaminase (Ast 产品)
- 别名
- Afu6g02490 antibody, Afu2g09650 antibody, aspartate transaminase antibody, Aspartate transaminase antibody, Aspartate transaminase, putative antibody, aspB antibody, CNM00360 antibody, AFUA_6G02490 antibody, AFUA_2G09650 antibody, AAT_1 antibody, Dd586_1627 antibody, aspB3 antibody, Fbal_1917 antibody, Emin_0749 antibody, CGB_M0340W antibody, Sgly_0435 antibody, Weevi_1216 antibody, Selsp_1595 antibody, Trebr_2143 antibody
- 背景
- Anti-Aspartate Transaminase antibody is present in the cytoplasm of cells, belonging to the class-I pyridoxal-phosphate-dependent aminotransferase family. Playing an active role in amino acid metabolism, Anti-Aspartate Transaminase antibody is ideal for investigators interested in Metabolism, Cancer, and Signal Transduction research. Synonyms: Aspartate aminotransferase 1 antibody, Aspartate aminotransferase cytoplasmic antibody, Aspartate aminotransferase cytosolic antibody, ec 2.6.1.1 antibody, GIG 18 antibody, GIG18 antibody, Glutamate oxaloacetate transaminase 1 antibody
- 基因ID
- 396967
- UniProt
- P00503
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