Urease 抗体 (Biotin)
URE
适用: Jack Bean
WB, ELISA
宿主: 兔
Polyclonal
Biotin
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- 抗原 See all Urease (URE) products
- Urease (URE)
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适用
- Jack Bean
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Urease antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), ELISA
- 交叉反应 （详细）
- Cross reactivities against Urease from other sources may occur but have not been specifically determined.
- 纯度
- Anti-Urease antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Urease [Jack Bean].
- 内毒素水平
- Low Endotoxin : No
- 免疫原
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Urease [Jack Bean]
Immunogen Type: Native Protein - 亚型
- IgG
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anti-Urease (URE) antibody
URE 适用: Jack Bean WB, ELISA, IP 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Anti-Urease antibody has been assayed against 1.0 µg of Urease in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4.000 to 1:20.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:20.000
Western Blot Dilution: 1:500 - 1:5.000 - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
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0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free - 注意事项
- Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
- 有效期
- Expiration date is one (1) year from date of opening.
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- 抗原
- Urease (URE)
- 别名
- Urease (URE 产品)
- 别名
- F12B7.10 antibody, F12B7_10 antibody, urease antibody, urease antibody, URE antibody, CNH01900 antibody
- 背景
- Urease is a protein that is commonly secreted from the bacterium H. Pylori and is integral to the immune response within the gastric system. Urease carries out a key enzymatic reaction in converting urea into free ammonia and carbonic acid. Urease is commonly derived from the Jack Bean plant in scientific research as the amino acid sequences are highly conserved between species and it is an abundant source of the protein. Anti-Urease Antibody is ideal for investigators in Immunology, Enzymology, and Cell Biology research. Synonyms: Urea Amidohydrolase antibody
- UniProt
- P07374
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