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Trypsinogen 抗体 (Biotin)

Rockland 200-4680-0100 适用: Cow WB, ELISA, DB 宿主: 兔 Polyclonal Biotin
Rockland
产品编号 ABIN1607813
Supplier Product No.: 200-4680-0100
发货至: 中国
  • 抗原 See all Trypsinogen products
    Trypsinogen
    适用
    Cow
    宿主
    • 5
    克隆类型
    • 5
    多克隆
    标记
    • 2
    • 2
    • 1
    This Trypsinogen antibody is conjugated to Biotin
    应用范围
    • 5
    • 5
    • 3
    • 2
    • 1
    Western Blotting (WB), ELISA, Dot Blot (DB)
    Supplier Product No.
    200-4680-0100
    Supplier
    Rockland
    原理
    Trypsinogen Antibody Biotin Conjugated
    交叉反应 (详细)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Trypsinogen [Bovine Pancreas].
    产品特性
    Synonyms: rabbit anti-Trypsinogen Antibody biotin Conjugation, biotin Conjugated rabbit anti-Trypsinogen Antibody, Brain trypsinogen antibody, Cationic trypsinogen antibody, Mesotrypsinogen antibody, Pancreatic anionic trypsinogen antibody, Protease, serine, 2 antibody, PRSS2 antibody
    纯化方法
    Anti-Trypsinogen antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
    免疫原

    Immunogen: Trypsinogen [Bovine Pancreas]

    Immunogen Type: Native Protein

    亚型
    IgG
  • 应用备注

    Application Note: Anti-Trypsinogen Biotin Conjugated antibody has been tested by dot blot and is suitable to be assayed against 1.0 μg of Trypsinogen in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.

    Western Blot Dilution: 1:500 - 1:3,000

    ELISA Dilution: 1:5,000 - 1:25,000

    Other: User Optimized

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    10 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    有效期
    12 months
  • 抗原
    Trypsinogen
    Abstract
    Trypsinogen 产品
    背景
    Background: Trypsinogen is the precursor form or zymogen of the pancreatic enzyme trypsin. It is found in pancreatic juice, along with amylase, lipase, and chymotrypsinogen. It is activated by enteropeptidase, which is found in the intestinal mucosa, to form trypsin. Once activated, the trypsin can activate more trypsinogen into trypsin. Trypsin cleaves the peptide bond on the carboxyl side of basic amino acids such as arginine and lysine. Anti-Trypsinogen antibody is ideal investigator involved in cytokines and growth factor research.
    基因ID
    282603
    UniProt
    Q29463
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