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- 抗原 See all Trypsin Inhibitor products
- Trypsin Inhibitor
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适用
- Soybean
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Trypsin Inhibitor antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), ELISA
- 交叉反应 （详细）
- Cross reactivity against Trypsin Inhibitor from other sources is unknown.
- 纯度
- Anti-Trypsin Inhibitor antibody is an IgG fraction purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Trypsin Inhibitor [Soy Bean].
- 内毒素水平
- Low Endotoxin : No
- 免疫原
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Anti-Trypsin Inhibitor was produced by repeated immunizations with soy bean trypsin inhibitor.
Immunogen Type: Native Protein - 亚型
- IgG
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anti-Trypsin Inhibitor antibody (HRP)
适用: Soybean WB, ELISA 宿主: 兔 Polyclonal HRP
anti-Trypsin Inhibitor antibody (Biotin)适用: Lima bean WB, ELISA 宿主: 兔 Polyclonal Biotin
anti-Trypsin Inhibitor antibody适用: Soybean ELISA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Anti-Trypsin Inhibitor antibody is suitable for western blotting and for capture ELISA. Researchers should determine optimal titers for applications that are not stated below.
ELISA Dilution: 1:5.000 - 1:25.000
Western Blot Dilution: 1:500 - 1:3.000 - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 10 mg/mL
- 缓冲液
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0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free - 注意事项
- Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
- 有效期
- Expiration date is one (1) year from date of opening.
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- 抗原
- Trypsin Inhibitor
- Abstract
- Trypsin Inhibitor 产品
- 别名
- CRISP11 antibody, LCRISP2 antibody, GRMZM2G447785 antibody, cysteine rich secretory protein LCCL domain containing 2 antibody, trypsin inhibitor antibody, trophoblast Kunitz domain protein 1 antibody, Kazal-type serine proteinase inhibitor 1 antibody, Bowman-Birk type bran trypsin inhibitor antibody, CRISPLD2 antibody, LOC548021 antibody, TKDP1 antibody, Spi1 antibody, LOC100284348 antibody
- 背景
- Anti-Trypsin Inhibitor detects trypsin inhibitor from soy bean. Soybean trypsin inhibitor inhibits trypsin and to a lesser extent chymotrypsin and plasmin. Soybean trypsin inhibitor will also inhibit other proteases by a mechanism similar to trypsin. It has inhibitory effects towards plasma kallikrein and coagulation Factor Xa. However, Soybean trypsin inhibitor will not inhibit metalloproteases, tissue-based kallikrein, acid proteases, or thio proteases. Anti-Trypsin Inhibitor Antibody is ideal for investigators involved in Cell Signaling, Biochemistry and Signal Transduction research. Synonyms: 25 kDa trypsin inhibitor (Protease inhibitor 15 preproprotein) antibody, CRISP8 antibody, KTi antibody, Kti S antibody, KTi3 antibody, KtiS antibody, Kunitz trypsin inhibitor antibody, Kunitz type trypsin inhibitor A antibody
- 基因ID
- 547831
- UniProt
- P01070
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