Trypsin Inhibitor 抗体 (Biotin)
-
- 抗原 See all Trypsin Inhibitor products
- Trypsin Inhibitor
-
适用
- Soybean
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This Trypsin Inhibitor antibody is conjugated to Biotin
-
应用范围
- Western Blotting (WB), ELISA
- Supplier Product No.
- 200-4679-0100
- Supplier
- Rockland
- 原理
- Trypsin Inhibitor Antibody Biotin Conjugated
- 交叉反应 (详细)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Trypsin Inhibitor [Soy Bean].
- 产品特性
- Synonyms: rabbit anti-trypsin inhibitor antibody biotin conjugation, biotin conjugated rabbit anti-trypsin inhibitor antibody, 25 kDa trypsin inhibitor (Protease inhibitor 15 preproprotein) antibody, CRISP8 antibody, KTi antibody, Kti S antibody, KTi3 antibody, KtiS antibody, Kunitz trypsin inhibitor antibody, Kunitz type trypsin inhibitor A antibody
- 纯化方法
- Anti-Trypsin Inhibitor antibody is an IgG fraction purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- 免疫原
-
Immunogen: Anti-Trypsin Inhibitor was produced by repeated immunizations with soy bean trypsin inhibitor.
Immunogen Type: Native Protein
- 亚型
- IgG
-
-
- 应用备注
-
Application Note: Anti-Trypsin Inhibitor Biotin Conjugated antibody has been tested by ELISA and western blotting. Researchers should determine optimal titers for applications that are not stated below.
Western Blot Dilution: 1:500 - 1:2,500
ELISA Dilution: 1:1,000 - 1:5,000
Other: User Optimized
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
-
Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 10 mg/mL
- 缓冲液
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
-
- 抗原
- Trypsin Inhibitor
- Abstract
- Trypsin Inhibitor 产品
- 背景
- Background: Anti-Trypsin Inhibitor detects trypsin inhibitor from soy bean. Soybean trypsin inhibitor inhibits trypsin and to a lesser extent chymotrypsin and plasmin. Soybean trypsin inhibitor will also inhibit other proteases by a mechanism similar to trypsin. It has inhibitory effects towards plasma kallikrein and coagulation Factor Xa. However, Soybean trypsin inhibitor will not inhibit metalloproteases, tissue-based kallikrein, acid proteases, or thio proteases. Anti-Trypsin Inhibitor Antibody is ideal for investigators involved in Cell Signaling, Biochemistry and Signal Transduction research.
- 基因ID
- 547831
- UniProt
- P01070
-