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ASRGL1 抗体 (Biotin)

ASRGL1 适用: E. coli WB, ELISA 宿主: 兔 Polyclonal Biotin
产品编号 ABIN1607805
发货至: 中国
  • 抗原 See all ASRGL1 抗体
    ASRGL1 (Asparaginase Like 1 (ASRGL1))
    适用
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    E. coli
    宿主
    • 15
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    克隆类型
    • 16
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    多克隆
    标记
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    This ASRGL1 antibody is conjugated to Biotin
    应用范围
    • 25
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    Western Blotting (WB), ELISA
    交叉反应 (详细)
    Cross reactivity against Asparaginase from other sources may occur but have not been specifically determined.
    纯度
    Anti-L-Asparaginase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Asparaginase [E. coli].
    内毒素水平
    Low Endotoxin : No
    免疫原
    Asparaginase [E. coli]
    Immunogen Type: Native Protein
    亚型
    IgG
    Top Product
    Discover our top product ASRGL1 Primary Antibody
  • 应用备注
    Anti-L-Asparaginase has been assayed against 1.0 µg of Asparaginase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:3.200 to 1:12,800 of the reconstitution concentration is suggested for this product.
    ELISA Dilution: 1:5.000 - 1:25.000
    Western Blot Dilution: 1:500 - 1:3.000
    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    10 mg/mL
    缓冲液
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
    注意事项
    Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
    储存条件
    -20 °C
    储存方法
    Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
    有效期
    Expiration date is one (1) year from date of opening.
  • 抗原
    ASRGL1 (Asparaginase Like 1 (ASRGL1))
    别名
    L-Asparaginase (ASRGL1 产品)
    别名
    ASRGL1 antibody, ALP antibody, ALP1 antibody, CRASH antibody, 2410004D18Rik antibody, AU040643 antibody, AW060375 antibody, Gliap antibody, Hiob2 antibody, zgc:103568 antibody, asparaginase like 1 antibody, L-asparaginase antibody, isoaspartyl peptidase/L-asparaginase antibody, ASRGL1 antibody, STY0886 antibody, ansA antibody, ECA0132 antibody, LOC5580113 antibody, EcHS_A0886 antibody, LACBIDRAFT_317700 antibody, BCAM1291 antibody, ZPR_0296 antibody, PAAG_01206 antibody, asrgl1 antibody, Asrgl1 antibody
    背景
    Anti-L-Asparaginase antibody recognizes the protein asparaginase. Asparaginase is responsible for the catalysis of aspargine to L-aspartic acid and ammonia. In food processing, asaparaginase is used to prevent the formation of acrylamide, a carcinogen, from the heating of asparagine which causes it to undergo the maillard reaction. Asparaginase is also used in cancer therapy since leukemic cells are unable to produce their own asparagine and therefore, must rely on circulating asparagine. Treatment with asparaginase converts the asparagine to aspartic acid denying the leukemic cells of asparagine, resulting in their death. Anti-L-Asparaginase antibody is suitable to investigators interested in cancer, metabolism, and enzymology. Synonyms: L ASNase II antibody, AnsA antibody, AnsB antibody, Colaspase antibody, Cytoplasmic asparaginase I antibody, L ASNase I antibody, L asparaginase II precursor antibody, L asparagine amidohydrolase I antibody
    基因ID
    1039460
    UniProt
    P00805
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