AnsB 抗体 (Biotin)
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- 抗原
- AnsB
- 适用
- E. coli
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- Biotin
- 应用范围
- ELISA, Western Blotting (WB), Dot Blot (DB)
- Supplier Product No.
- 200-4671-0100
- Supplier
- Rockland
- 原理
- L-Asparaginase Antibody Biotin Conjugated
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Asparaginase [E.
- 产品特性
- Synonyms: rabbit anti-L-Asparaginase Antibody biotin Conjugation, biotin Conjugated rabbit anti-L-Asparaginase Antibody, L ASNase II antibody, AnsA antibody, AnsB antibody, Colaspase antibody, Cytoplasmic asparaginase I antibody, L ASNase I antibody, L asparaginase II precursor antibody, L asparagine amidohydrolase I antibody
- 纯化方法
- Anti-L-Asparaginase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- 免疫原
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Immunogen: Asparaginase [E. coli]
Immunogen Type: Native Protein
- 亚型
- IgG
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anti-AnsB antibody (HRP)
适用: E. coli ELISA, WB 宿主: 兔 Polyclonal HRP
anti-AnsB antibody适用: E. coli ELISA, WB, IHC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Application Note: Anti-L-Asparaginase Biotin Conjugated Antibody has been by ELISA, dot blot, and western blot. This product is assayed against 1.0 μg of Asparaginase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:3,200 to 1:12,800 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500
ELISA Dilution: 1:1,000
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 10 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- AnsB
- 背景
- Background: Anti-L-Asparaginase antibody recognizes the protein asparaginase. Asparaginase is responsible for the catalysis of asparagine to L-aspartic acid and ammonia. In food processing, asparaginase is used to prevent the formation of acrylamide, a carcinogen, from the heating of asparagine which causes it to undergo the maillard reaction. Asparaginase is also used in cancer therapy since leukemic cells are unable to produce their own asparagine and therefore, must rely on circulating asparagine. Treatment with asparaginase converts the asparagine to aspartic acid denying the leukemic cells of asparagine, resulting in their death. Anti-L-Asparaginase antibody is suitable to investigators interested in cancer, metabolism, and enzymology.
- 基因ID
- 1039460
- UniProt
- P00805
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