CES3 抗体 (Biotin)
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- 抗原 See all CES3 抗体
- CES3 (Carboxylesterase 3 (CES3))
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适用
- Pig
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This CES3 antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), ELISA
- Supplier Product No.
- 200-4669-0100
- Supplier
- Rockland
- 原理
- Esterase Antibody Biotin Conjugated
- 交叉反应 (详细)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Esterase [Porcine Liver].
- 产品特性
- Synonyms: rabbit anti-Esterase Antibody biotin Conjugation, biotin conjugated rabbit anti-Esterase Antibody, Liver carboxylesterase, Proline-beta-naphthylamidase, Retinyl ester hydrolase
- 纯化方法
- Anti-Esterase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- 免疫原
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Immunogen: Esterase [Porcine Liver]
Immunogen Type: Native Protein
- 亚型
- IgG
- Top Product
- Discover our top product CES3 Primary Antibody
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- 应用备注
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Application Note: Anti-Esterase Biotin Conjugated Antibody has been tested by ELISA and western blot. This product is assayed against 1.0 μg of Esterase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: User Optimized
ELISA Dilution: 1:2,000 - 1:5,000
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 10 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- CES3 (Carboxylesterase 3 (CES3))
- 别名
- CES3 (CES3 产品)
- 背景
- Background: Anti-Esterase antibody is involved in the detoxification of xenobiotics, as well as in the activation of ester and amide prodrugs. The antibody is inactive on long-chain triacylglycerols and diacylglycerol, but active towards triacylglycerides composed with short-chain fatty acids from C2 to C6 and monoacylglycerols containing fatty acids from C2 to C12. Esterase hydrolyzes aromate and alkyl esters and vitamin A acetate, the rate of hydrolysis varies due to the hydrolyzing substances. Anti-Esterase antibody member of the type-B carboxylesterase/lipase family.
- 基因ID
- 100626873, 343791015
- UniProt
- Q29550
- 途径
- Monocarboxylic Acid Catabolic Process
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