Hexokinase 2 抗体 (Biotin)
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- 抗原 See all Hexokinase 2 (HK2) 抗体
- Hexokinase 2 (HK2)
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适用
- Saccharomyces cerevisiae
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Hexokinase 2 antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), ELISA
- Supplier Product No.
- 200-4659-0100
- Supplier
- Rockland
- 原理
- Hexokinase Antibody Biotin Conjugated
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Hexokinase [Yeast].
- 产品特性
- Synonyms: rabbit anti-Hexokinase Antibody Biotin Conjugation, Biotin Conjugated rabbit anti-Hexokinase Antibody, DKFZp686M1669 antibody, Hexokinase 2 antibody, Hexokinase 2 muscle antibody, Hexokinase type II antibody
- 纯化方法
- Anti-Hexokinase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- 免疫原
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Immunogen: Hexokinase [Yeast]
Immunogen Type: Native Protein
- 亚型
- IgG
- Top Product
- Discover our top product HK2 Primary Antibody
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anti-Hexokinase 2 (HK2) (AA 1-120) antibody
HK2 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Hexokinase 2 (HK2) antibodyHK2 适用: 人, 犬 WB, IF, FACS 宿主: 小鼠 Monoclonal 4C5 unconjugated
anti-Hexokinase 2 (HK2) (AA 818-917) antibodyHK2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4H1 unconjugated
anti-Hexokinase 2 (HK2) antibodyHK2 适用: 人 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 3D3 unconjugated
anti-Hexokinase 2 (HK2) antibodyHK2 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Hexokinase 2 (HK2) (C-Term) antibodyHK2 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Hexokinase 2 (HK2) antibodyHK2 适用: 人 WB, IHC, ELISA, IF 宿主: 小鼠 Monoclonal 3D3 unconjugated
anti-Hexokinase 2 (HK2) (AA 469-669) antibodyHK2 适用: 人 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Hexokinase 2 (HK2) (AA 453-483) antibodyHK2 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB5333 unconjugated
anti-Hexokinase 2 (HK2) (AA 334-526) antibodyHK2 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Application Note: Anti-Hexokinase Biotin Conjugated Antibody has been tested by ELISA and western blot. This product is assayed against 1.0 μg of Hexokinase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:1,000
ELISA Dilution: 1:1,000 - 1:2,500
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 10 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- Hexokinase 2 (HK2)
- 别名
- HXK2 (HK2 产品)
- 背景
- Background: Anti-Hexokinase antibody recognizes hexokinase which phosphorylates hexoses. Hexokinase uses one ATP molecule when phosphorylating six-carbon sugars. In mammals there are four isozymes of hexokinase, the most notable of which is hexokinase IV, also known as glucokinase which is highly specific for glucose. Phosphorylation of hexoses commit them to certain metabolic pathways. For example, phosphorylation of glucose to glucose-6-phosphate limits it to glycolysis or glycogen synthesis. Anti-Hexokinase antibody is ideal for investigators interested in Metabolism, Cardiology, or Enzymology.
- 基因ID
- 852639
- UniProt
- P04806
- 途径
- PI3K-Akt Signaling, Carbohydrate Homeostasis, Warburg Effect
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