Hexokinase 抗体 (Biotin)
适用: Saccharomyces cerevisiae
WB, ELISA
宿主: 兔
Polyclonal
Biotin
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- 抗原 See all Hexokinase 抗体
- Hexokinase
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适用
- Saccharomyces cerevisiae
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Hexokinase antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), ELISA
- 交叉反应 （详细）
- Cross reactivity against Hexokinase from other sources may occur but have not been specifically determined.
- 纯度
- Anti-Hexokinase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Hexokinase [Yeast].
- 内毒素水平
- Low Endotoxin : No
- 免疫原
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Hexokinase [Yeast]
Immunogen Type: Native Protein - 亚型
- IgG
- Top Product
- Discover our top product Hexokinase Primary Antibody
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anti-Hexokinase (AA 1-917) antibody
适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4D7 unconjugated
anti-Hexokinase antibody (HRP)适用: Saccharomyces cerevisiae WB, ELISA 宿主: 兔 Polyclonal HRP
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- 应用备注
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Anti-Hexokinase has been assayed against 1.0 µg of Hexokinase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4.000 to 1:20.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:4.000 - 1:20.000
Western Blot Dilution: 1:500 - 1:2.000 - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 10 mg/mL
- 缓冲液
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0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free - 注意事项
- Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
- 有效期
- Expiration date is one (1) year from date of opening.
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- 抗原
- Hexokinase
- Abstract
- Hexokinase 产品
- 背景
- Anti-Hexokinase antibody recognizes hexokinase which phosphorylates hexoses. Hexokinase uses one ATP molecule when phosphorylating six-carbon sugars. In mammals there are four isozymes of hexokinase, the most notable of which is hexokinase IV, also known as glucokinase which is highly specific for glucose. Phosporylation of hexoses commit them to certain metabolic pathways. For example, phosphorylation of glucose to glucose-6-phosphate limits it to glycolysis or glycogen synthesis. Anti-Hexokinase antibody is ideal for investigators interested in Metabolism, Cardiology, or Enzymology. Synonyms: DKFZp686M1669 antibody, Hexokinase 2 antibody, Hexokinase 2 muscle antibody, Hexokinase type II antibody
- 基因ID
- 852639
- UniProt
- P04807
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