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CPA 抗体 (Biotin)

CPA 适用: Cow WB, ELISA 宿主: 兔 Polyclonal Biotin
产品编号 ABIN1607785
发货至: 中国
  • 抗原 See all CPA products
    CPA (Carboxypeptidase A (CPA))
    适用
    • 6
    • 1
    • 1
    Cow
    宿主
    • 7
    克隆类型
    • 7
    多克隆
    标记
    • 4
    • 2
    • 1
    This CPA antibody is conjugated to Biotin
    应用范围
    • 6
    • 5
    • 3
    • 2
    • 1
    Western Blotting (WB), ELISA
    交叉反应 (详细)
    Cross reactivity against Carboxypeptidase A from other sources may occur but have not been specifically determined.
    纯度
    Anti-Carboxypeptidase A is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Carboxypeptidase A [Bovine Pancreas].
    内毒素水平
    Low Endotoxin : No
    免疫原
    Carboxypeptidase A [Bovine Pancreas]
    Immunogen Type: Native Protein
    亚型
    IgG
  • 应用备注
    Anti-Carboxypeptidase A has been assayed against 1.0 µg of Carboxypeptidase A in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:15.000 to 1:55.000 of the reconstitution concentration is suggested for this product.
    ELISA Dilution: 1:5.000 - 1:25.000
    Western Blot Dilution: 1:500 - 1:3.000
    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    10 mg/mL
    缓冲液
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
    注意事项
    Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
    储存条件
    -20 °C
    储存方法
    Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
    有效期
    Expiration date is one (1) year from date of opening.
  • 抗原
    CPA (Carboxypeptidase A (CPA))
    别名
    Carboxypeptidase A (CPA 产品)
    背景
    Anti-Carboxypeptidase A antibody catalyzes the release of C-terminal amino acids with little or no action with –Asp, -Glu, -Arg, -Lys or –Pro. This antibody is ideal for investigators interested in Metabolism, Cancer, Signal Transduction, or Cell Biology research. Synonyms: Carboxypeptidase A1 precursor antibody, CPA1 antibody, Pancreatic carboxypeptidase A1 antibody, Procarboxypeptidase A1 pancreatic antibody
    基因ID
    286762
    UniProt
    P00730
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