CPA 抗体 (Biotin)
CPA
适用: Cow
WB, ELISA
宿主: 兔
Polyclonal
Biotin
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- 抗原 See all CPA products
- CPA (Carboxypeptidase A (CPA))
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适用
- Cow
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This CPA antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), ELISA
- 交叉反应 （详细）
- Cross reactivity against Carboxypeptidase A from other sources may occur but have not been specifically determined.
- 纯度
- Anti-Carboxypeptidase A is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Carboxypeptidase A [Bovine Pancreas].
- 内毒素水平
- Low Endotoxin : No
- 免疫原
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Carboxypeptidase A [Bovine Pancreas]
Immunogen Type: Native Protein - 亚型
- IgG
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anti-Carboxypeptidase A (CPA) antibody
CPA 适用: Cow WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Carboxypeptidase A (CPA) antibodyCPA 适用: 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Anti-Carboxypeptidase A has been assayed against 1.0 µg of Carboxypeptidase A in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:15.000 to 1:55.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:25.000
Western Blot Dilution: 1:500 - 1:3.000 - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 10 mg/mL
- 缓冲液
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0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free - 注意事项
- Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
- 有效期
- Expiration date is one (1) year from date of opening.
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- 抗原
- CPA (Carboxypeptidase A (CPA))
- 别名
- Carboxypeptidase A (CPA 产品)
- 背景
- Anti-Carboxypeptidase A antibody catalyzes the release of C-terminal amino acids with little or no action with –Asp, -Glu, -Arg, -Lys or –Pro. This antibody is ideal for investigators interested in Metabolism, Cancer, Signal Transduction, or Cell Biology research. Synonyms: Carboxypeptidase A1 precursor antibody, CPA1 antibody, Pancreatic carboxypeptidase A1 antibody, Procarboxypeptidase A1 pancreatic antibody
- 基因ID
- 286762
- UniProt
- P00730
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