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Ascorbate Oxidase 抗体 (Biotin)

AO 适用: Pumpkin ELISA, WB, IP 宿主: 兔 Polyclonal Biotin
产品编号 ABIN1607781
发货至: 中国
  • 抗原 See all Ascorbate Oxidase (AO) products
    Ascorbate Oxidase (AO)
    适用
    • 7
    • 2
    Pumpkin
    宿主
    • 9
    克隆类型
    • 9
    多克隆
    标记
    • 5
    • 3
    • 1
    This Ascorbate Oxidase antibody is conjugated to Biotin
    应用范围
    • 8
    • 8
    • 3
    • 2
    • 2
    • 2
    ELISA, Western Blotting (WB), Immunoprecipitation (IP)
    交叉反应 (详细)
    Cross reactivity against Ascorbate Oxidase from other sources may occur but have not been specifically determined.
    纯度
    Anti-Ascorbate Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Ascorbate Oxidase [cucurbita].
    内毒素水平
    Low Endotoxin : No
    免疫原
    Ascorbate Oxidase [cucurbita]
    Immunogen Type: Native Protein
    亚型
    IgG
  • 应用备注
    Anti-Ascorbate Oxidase has been assayed against 1.0 µg of Ascorbate Oxidase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:2.000 to 1:10.000 of the reconstitution concentration is suggested for this product.
    ELISA Dilution: 1:5.000 - 1:20.000
    IF Immunoprecipitation Dilution: 1:100
    Western Blot Dilution: 1:500 - 1:5.000
    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    10 mg/mL
    缓冲液
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
    注意事项
    Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
    储存条件
    -20 °C
    储存方法
    Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
    有效期
    Expiration date is one (1) year from date of opening.
  • 抗原
    Ascorbate Oxidase (AO)
    别名
    Ascorbate Oxidase (AO 产品)
    别名
    AO antibody, ascorbate oxidase antibody, L-ascorbate oxidase antibody, AO antibody, LOC9310099 antibody
    背景
    Anti-Ascorbate Oxidase antibody is cytoplasmic and performs binding with copper ions. Anti-Ascorbate Oxidase belongs to the multicopper oxidase family and may be a factor in the redox system involving ascorbic acid, acting as a catalyst. Found prevalently in plants, Ascorbate Oxidase has been suggested to increase plant growth. Anti-Ascorbate Oxidase antibody is ideal for researchers interested in the investigation of Cell Biology. Synonyms: L-ascorbate oxidase Ascorbase ASO EC=1.10.3.3
    UniProt
    P24792
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