Amylase, alpha 抗体 (Biotin)
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- 抗原 See all Amylase, alpha (AMY) 抗体
- Amylase, alpha (AMY)
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适用
- Bacillus amyloliquefaciens
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Amylase, alpha antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), ELISA, Immunoprecipitation (IP)
- Supplier Product No.
- 200-4645-0100
- Supplier
- Rockland
- 原理
- Alpha Amylase Antibody Biotin Conjugated
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified a-Amylase [B.
- 产品特性
- Synonyms: rabbit anti-Alpha Amylase Antibody biotin Conjugation, biotin conjugated rabbit anti-Alpha Amylase Antibody, Alpha-amylase, 1,4-alpha-D-glucan glucanohydrolase
- 纯化方法
- Anti-Alpha Amylase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- 免疫原
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Immunogen: a-Amylase [Bacillus amyloliquefaciens]
Immunogen Type: Native Protein
- 亚型
- IgG
- Top Product
- Discover our top product AMY Primary Antibody
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anti-Amylase, alpha (AMY) antibody
AMY 适用: Oryza sativa WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Application Note: Anti-Alpha Amylase Biotin Conjugated Antibody has been tested by western blot and is suitable to be assayed against 1.0 μg of a-Amylase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:5,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:5,000 - 1:20,000
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 10 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- Amylase, alpha (AMY)
- 别名
- Alpha-amylase (AMY 产品)
- 背景
- Background: Alpha Amylase is a member of the glycosyl hydrolase 13 family, involved primarily in the hydrolysis of biopolymer residues of 1, 4-alpha-D-glucosidic linkages in polysaccharides. It performs its catalytic function in metabolism of carbohydrates. Anti-Alpha Amylase antibody is ideal for investigators involved in Metabolism, Signal Transduction, and Cancer research.
- 基因ID
- 7849308
- NCBI登录号
- WP_013352208
- UniProt
- P00692
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