Amylase, alpha 抗体 (Biotin)
AMY
适用: Bacillus amyloliquefaciens
WB, ELISA, IP
宿主: 兔
Polyclonal
Biotin
1 image
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- 抗原 See all Amylase, alpha (AMY) 抗体
- Amylase, alpha (AMY)
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适用
- Bacillus amyloliquefaciens
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Amylase, alpha antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), ELISA, Immunoprecipitation (IP)
- 交叉反应 （详细）
- Cross reactivity against a-Amylase from other sources may occur but has not been specifically determined.
- 纯度
- Anti-Alpha Amylase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified a-Amylase [B.amyloliquefaciens].
- 内毒素水平
- Low Endotoxin : No
- 免疫原
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a-Amylase [Bacillus amyloliquefaciens]
Immunogen Type: Native Protein - 亚型
- IgG
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anti-Amylase, alpha (AMY) antibody
AMY 适用: Oryza sativa WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Anti-Alpha Amylase has been assayed against 1.0 µg of a-Amylase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4.000 to 1:20.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:20.000
IF Immunoprecipitation Dilution: 1:100
Western Blot Dilution: 1:500 - 1:5.000 - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 10 mg/mL
- 缓冲液
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0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free - 注意事项
- Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
- 有效期
- Expiration date is one (1) year from date of opening.
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- 抗原
- Amylase, alpha (AMY)
- 别名
- Alpha Amylase (AMY 产品)
- 别名
- LOC663954 antibody, LOC100136478 antibody, alpha amylase antibody, alpha-amylase antibody, LOC663954 antibody, HQ_RS05305 antibody, LOC100136478 antibody
- 背景
- Alpha Amylase is a member of the glycosyl hydrolase 13 family, involved primarily in the hydrolysis of biopolymer residues of 1, 4-alpha-D-glucosidic linkages in polysaccharides. It performs its catalytic function in metabolism of carbohydrates. Anti-Alpha Amylase antibody is ideal for investigators involved in Metabolism, Signal Transduction, and Cancer research. Synonyms: Alpha-amylase EC=3.2.1.1 1,4-alpha-D-glucan glucanohydrolase
- 基因ID
- 7849308
- UniProt
- P00692
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