Cross reactivity against Arginase from other sources is unknown.
纯度
Anti-Arginase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Arginase [Bovine Liver].
内毒素水平
Low Endotoxin : No
免疫原
Arginase [Bovine Liver] Immunogen Type: Native Protein
Anti-Arginase has been assayed against 1.0 µg of Arginase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1.000 to 1:4.000 of the reconstitution concentration is suggested for this product. ELISA Dilution: 1:5.000 - 1:25.000 Western Blot Dilution: 1:500 - 1:3.000
限制
仅限研究用
状态
Liquid
浓度
10 mg/mL
缓冲液
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
注意事项
Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
储存条件
-20 °C
储存方法
Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
有效期
Expiration date is one (1) year from date of opening.
Anti-Arginase antibody is localized in the cytoplasm and plays a key role in the Arginine Metabolism Urea Cycle. Anti-Arginase antibody, belonging to the arginase family, is ideal for researchers involved in Metabolism, Cancer, and Signal Transduction research. Synonyms: A I antibody, Al antibody, ARG 1 antibody, ARG1 antibody, Arginase 1 antibody, Arginase liver antibody, Arginase type I antibody, Arginase1 antibody, Liver type arginase antibody, Type I arginase antibody