Glucose Oxidase 抗体 (Biotin)
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- 抗原 See all Glucose Oxidase (GOD) products
- Glucose Oxidase (GOD)
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适用
- Aspergillus niger
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Glucose Oxidase antibody is conjugated to Biotin
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应用范围
- ELISA, Western Blotting (WB)
- Supplier Product No.
- 200-4637-0100
- Supplier
- Rockland
- 原理
- Glucose Oxidase Antibody Biotin Conjugated
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Glucose Oxidase [A.
- 产品特性
- Synonyms: rabbit anti-Glucose Oxidase Antibody biotin Conjugation, biotin Conjugated rabbit anti-Glucose Oxidase Antibody, Beta D Glucose Oxygen 1 Oxido Reductase antibody, Glucose oxidase (Precursor) antibody, Glucose Oxyhydrase antibody, GOD antibody
- 纯化方法
- Anti-GLUCOSE OXIDASE Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- 免疫原
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Immunogen: Glucose Oxidase [A.niger]
Immunogen Type: Native Protein
- 亚型
- IgG
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anti-Glucose Oxidase (GOD) antibody
GOD 适用: Aspergillus niger ELISA, WB, IHC 宿主: 山羊 Polyclonal unconjugated
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- 应用备注
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Application Note: Anti-GLUCOSE OXIDASE Biotin Conjugated Antibody has been tested by ELISA and Western blot. This product is assayed against 1.0 μg of Glucose Oxidase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:5,000 to 1:25,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: User Optimized
ELISA Dilution: User Optimized
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 10 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- Glucose Oxidase (GOD)
- 别名
- Glucose Oxidase (GOD 产品)
- 背景
- Background: Glucose Oxidase antibody detect the glucose oxidase protein. The glucose oxidase enzyme (GOx) (EC 1.1.3.4) is an oxido-reductase that catalyzes the oxidation of glucose to hydrogen peroxide and D-glucono-δ-lactone. In cells, it aids in breaking the sugar down into its metabolites. Glucose oxidase is widely used for the determination of free glucose in body fluids (diagnostics), in vegetal raw material, and in the food industry. GOx is a dimeric protein, the 3D structure of which has been elucidated. The active site where glucose binds is in a deep pocket. The enzyme, like many proteins that act outside of cells, is covered with carbohydrate chains. At pH 7, glucose exists in solution in cyclic hemiacetal form as 63.6 % β-D-glucopyranose and 36.4 % α-D-glucopyranose, the proportion of linear and furanose form being negligible. Anti-Glucose Oxidase antibody is ideal for investigators involved in glucose energy metabolism research.
- 基因ID
- 2683500
- UniProt
- P13006
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