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Calf Intestinal Alkaline Phosphatase (CIAP) 抗体 (Biotin)

Rockland 200-4635-0100 CIAP 适用: Cow WB, ELISA 宿主: 兔 Polyclonal Biotin
Rockland
产品编号 ABIN1607763
Supplier Product No.: 200-4635-0100
发货至: 中国
  • 抗原 See all Calf Intestinal Alkaline Phosphatase (CIAP) products
    Calf Intestinal Alkaline Phosphatase (CIAP)
    适用
    Cow
    宿主
    • 3
    • 2
    • 2
    克隆类型
    • 7
    多克隆
    标记
    • 4
    • 3
    Biotin
    应用范围
    • 7
    • 6
    • 4
    • 2
    Western Blotting (WB), ELISA
    Supplier Product No.
    200-4635-0100
    Supplier
    Rockland
    原理
    Alkaline Phosphatase Antibody Biotin Conjugated
    交叉反应 (详细)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Alkaline Phosphatase [Calf Intestine].
    产品特性
    Synonyms: rabbit anti-Alkaline Phosphatase Antibody biotin Conjugation, biotin conjugated rabbit anti-Alkaline Phosphatase Antibody, Alkaline phosphomonoesterase antibody, ALPI antibody, Glycerophosphatase antibody, IAP antibody, Intestinal alkaline phosphatase antibody, Kasahara isozyme antibody
    纯化方法
    This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
    免疫原

    Immunogen: Alkaline Phosphatase [Calf Intestine]

    Immunogen Type: Native Protein

    亚型
    IgG
  • 应用备注

    Application Note: Anti-Alkaline Phosphatase Biotin Conjugated Antibody has been tested by ELISA and Western blot. This product is assayed against 1.0 μg of Alkaline Phosphatase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.

    Western Blot Dilution: 1:500 - 1:2,000

    ELISA Dilution: 1:1,000 - 1:10,000

    Other: User Optimized

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    10 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    有效期
    12 months
  • 抗原
    Calf Intestinal Alkaline Phosphatase (CIAP)
    别名
    Calf Intenstinal Alkaline Phosphatase (CIAP 产品)
    背景
    Background: At a high pH , Anti-Alkaline Phosphatase antibodies strip phosphate groups from DNA, RNA, and proteins. ALP is most abundant in placental and intestinal tissue specific such as the liver, placenta, and bone. Located in the cell membrane, Anti-Alkaline Phosphatase antibodies are ideal for researchers involved in Cancer, Enzyme, Tags and Cell Marker, and Cell Cycle research.
    基因ID
    280993
    UniProt
    P19111
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