Ovalbumin 抗体 (Biotin)
-
- 抗原 See all Ovalbumin (OVA) 抗体
- Ovalbumin (OVA)
-
适用
- 小鸡
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This Ovalbumin antibody is conjugated to Biotin
-
应用范围
- Western Blotting (WB), ELISA, Immunoprecipitation (IP), Dot Blot (DB)
- Supplier Product No.
- 200-4633-0100
- Supplier
- Rockland
- 原理
- Ovalbumin Antibody Biotin Conjugated
- 交叉反应 (详细)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Ovalbumin [Hen Egg White].
- 产品特性
- Synonyms: rabbit anti-Ovalbumin Antibody biotin Conjugation, biotin conjugated rabbit anti-Ovalbumin antibody, Egg albumin antibody, Plakalbumin, Allergen Gal d II, Allergen=Gal d 2, Hen Egg White
- 纯化方法
- Anti-Ovalbumin Antibody Biotin Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- 免疫原
-
Immunogen: Ovalbumin [Hen Egg White]
Immunogen Type: Native Protein
- 亚型
- IgG
- Labeling Ratio
- 10-20
- Top Product
- Discover our top product OVA Primary Antibody
-
-
- 应用备注
-
Application Note: Anti-Ovalbumin Antibody Biotin Conjugated is tested by ELISA, dot blot, and western blotting. In western blots anti-ovalbumin detects a single band of the expected apparent molecular weight. Researchers should determine optimal titers for applications that are not stated below.
Western Blot Dilution: 1:500 - 1:2,500
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:1,000 - 1:5,000
Other: User Optimized
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
-
Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1 mg/mL
- 缓冲液
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
-
- 抗原
- Ovalbumin (OVA)
- 别名
- SERPINB14 (OVA 产品)
- 背景
- Background: Ovalbumin Antibody Biotin Conjugated is specific for ovalbumin. Ovalbumin (abbreviated OVA) is the main protein found in egg white, making up 60-65 % of the total protein. Ovalbumin displays sequence and three-dimensional homology to the serpin superfamily. The function of ovalbumin is unknown, although it is presumed to be a storage protein or carrier protein.
- 基因ID
- 396058
- UniProt
- P01012
-