Dextranase 抗体 (Biotin)
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- 抗原 See all Dextranase products
- Dextranase
- 适用
- Penicillium sp.
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Dextranase antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), ELISA, Immunoprecipitation (IP)
- 交叉反应 (详细)
- Cross reactivity against Dextranase from other sources is unknown.
- 纯度
- Anti-Dextranase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Dextranase [Penicillium species].
- 内毒素水平
- Low Endotoxin : No
- 免疫原
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Dextranase [Penicillium species] DEX
Immunogen Type: Native Protein - 亚型
- IgG
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- 应用备注
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Anti-Dextranase has been assayed against 1.0 µg of Dextranase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:3.000 to 1:14.000 of the reconstitution concentration is suggested for this product. This product has been assayed against 1.0 µg of Dextranase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:3.000 to 1:14.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:20.000
IF Immunoprecipitation Dilution: 1:100
Western Blot Dilution: 1:500 - 1:5.000 - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 10 mg/mL
- 缓冲液
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0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free - 注意事项
- Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
- 有效期
- Expiration date is one (1) year from date of opening.
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- 抗原
- Dextranase
- Abstract
- Dextranase 产品
- 别名
- dextranase antibody, Dextranase antibody, Teth39_0264 antibody, Teth514_0757 antibody, VDBG_04792 antibody, Thet_2161 antibody, Thebr_0271 antibody
- 背景
- Anti-Dextranase antibody is a transferase enzyme which catalyzes the synthesis of dextran by engaging in the endohydrolysis of 1, 6 alpha-D-glucosidic linkages in dextran. Belonging to the glycosyl hydrolase 49 family, Anti-Destranase antibody is ideal for researchers interested in Metabolism and Signal Transduction research. Synonyms: Dextranase Alpha-1,6-glucan-6-glucanohydrolase DEX
- UniProt
- P48845
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