Dextranase 抗体 (Biotin)
-
- 抗原 See all Dextranase products
- Dextranase
- 适用
- Penicillium sp.
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This Dextranase antibody is conjugated to Biotin
-
应用范围
- Western Blotting (WB), ELISA, Immunoprecipitation (IP)
- Supplier Product No.
- 200-406-089-0100
- Supplier
- Rockland
- 原理
- Dextranase (Penicillium Species) Antibody Biotin Conjugated
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Dextranase [Penicillium species].
- 产品特性
- Synonyms: rabbit anti-Dextranase Antibody biotin Conjugation, biotin Conjugated rabbit anti-Dextranase Antibody, dextranase, Alpha-1,6-glucan-6-glucanohydrolase, DEX
- 纯化方法
- Anti-Dextranase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- 免疫原
-
Immunogen: Dextranase [Penicillium species] DEX
Immunogen Type: Native Protein
- 亚型
- IgG
-
anti-Dextranase antibody
适用: Penicillium sp. WB, ELISA, IP 宿主: 兔 Polyclonal unconjugated
-
- 应用备注
-
Application Note: Anti-Dextranase Biotin Antibody has been tested by ELISA and western blot and is assayed against 1.0 μg of Dextranase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:14,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:5,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:2,000 - 1:10,000
Other: User Optimized
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
-
Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 10 mg/mL
- 缓冲液
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
-
- 抗原
- Dextranase
- Abstract
- Dextranase 产品
- 背景
- Background: Anti-Dextranase antibody is a transferase enzyme which catalyzes the synthesis of dextran by engaging in the endohydrolysis of 1, 6 alpha-D-glucosidic linkages in dextran. Belonging to the glycosyl hydrolase 49 family, Anti-Dextranase antibody is ideal for researchers interested in Metabolism and Signal Transduction research.
- UniProt
- P48845
-
