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AKT1 抗体 (Internal Region)

Rockland 200-301-j34l AKT1 适用: 人 WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 5E5-F5-D7 unconjugated
Rockland
产品编号 ABIN1607688
Supplier Product No.: 200-301-j34l
发货至: 中国
  • 抗原 See all AKT1 抗体
    AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))
    抗原表位
    • 80
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    Internal Region
    适用
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    宿主
    • 404
    • 89
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    小鼠
    克隆类型
    • 368
    • 134
    单克隆
    标记
    • 286
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    This AKT1 antibody is un-conjugated
    应用范围
    • 396
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    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)
    Supplier Product No.
    200-301-j34l
    Supplier
    Rockland
    原理
    AKT1 Antibody
    交叉反应 (详细)
    Anti-AKT1 antibody is directed against human AKT1. The antibody detects both unphosphorylated and phosphorylated forms of the protein.
    产品特性
    Synonyms: mouse anti-AKT1 antibody, AKT-1, PKB antibody, PKB gamma antibody, PKBGAMMA antibody, PRKBG antibody, Protein kinase Akt 1 antibody, Protein kinase B gamma antibody, RAC-gamma serine/threonine-protein kinase, RAC-PK-gamma
    过滤
    Sterile filtered
    免疫原

    Immunogen: Anti-AKT1 Antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT1 protein.

    Immunogen Type: Conjugated Peptide

    克隆位点
    5E5-F5-D7
    亚型
    IgG2b kappa
    Top Product
    Discover our top product AKT1 Primary Antibody
  • 应用备注

    Flow Cytometry Dilution: User Optimized

    Immunohistochemistry Dilution: 20 μg/mL

    Application Note: Anti-AKT1 Antibody is tested in ELISA, Flow Cytometry, and western blotting. This antibody is suitable in immunohistochemistry. Expect a band approximately 56 kDa in size corresponding to AKT1 protein by western blotting in the appropriate cell lysate or extract. This monoclonal antibody reacts with human AKT. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry we recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative. No pre-treatment of sample is required.

    Western Blot Dilution: 1:500-1:2000

    ELISA Dilution: 1:2,000 - 1:10,000

    Other: User Optimized

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: None
    储存液
    Without preservative
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    有效期
    12 months
  • 抗原
    AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))
    别名
    AKT1 (AKT1 产品)
    背景
    Background: AKT1 Antibody detects AKT1 which is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis, (ii) promotion of proliferation. Anti-AKT1 Antibody is ideal for investigators involved in Cell Signaling, Neuroscience and Signal Transduction research.
    UniProt
    P31749
    途径
    PI3K-Akt Signaling, RTK signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling, Warburg Effect
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