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IdU 抗体

IdU 适用: 化学剂 IHC, WB, ELISA, FM, FACS 宿主: 小鼠 Monoclonal 32D8-D9 unconjugated
产品编号 ABIN1607683
发货至: 中国
  • 抗原 See all IdU products
    IdU (Iododeoxyuridine (IdU))
    适用
    • 6
    • 5
    化学剂
    宿主
    • 8
    • 3
    小鼠
    克隆类型
    • 11
    单克隆
    标记
    • 11
    This IdU antibody is un-conjugated
    应用范围
    Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Fluorescence Microscopy (FM), Flow Cytometry (FACS)
    交叉反应 (详细)
    Cross reactivity is not observed with BrdU, CldU or FdU.
    纯度
    Anti-IdU Monoclonal Antibody was purified from ascites fluid by Protein A chromatography. This antibody is specific for IdU.
    内毒素水平
    Low Endotoxin : No
    免疫原
    Anti-IdU mononclonal antibody was produced in mice by repeated immunizations prepared via immunizations with BromodeoxyUridine-KLH followed by hybridoma development.
    Immunogen Type: Other
    克隆位点
    32D8-D9
  • 应用备注
    Anti-IdU Antibody has been tested as suitable for western blot, ELISA, and immunofluorescence microscopy. This antibody may be suitable for additional immunoassays including flow cytometry and immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Expect to detect incoporated IdU thymidine analog from replicated cells.
    ELISA Dilution: 1:1000-1:10000
    Western Blot Dilution: 1:2000 - 1:5000
    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1 mg/mL
    缓冲液
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    储存条件
    4 °C/-20 °C
    储存方法
    Store IdU Antibody at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.
    有效期
    Expiration date is one (1) year from date of opening.
  • Alén, Gómez-Redondo, Rivera, Suárez, Ramos-Ibeas, Pericuesta, Fernández-González, Perez-Cerezales, Horiuchi, Orio, Rodriguez de Fonseca, Gutiérrez-Adán: "Sex-Dimorphic Behavioral Alterations and Altered Neurogenesis in U12 Intron Splicing-Defective Zrsr1 Mutant Mice." in: International journal of molecular sciences, Vol. 20, Issue 14, (2019) (PubMed).

  • 抗原
    IdU (Iododeoxyuridine (IdU))
    Abstract
    IdU 产品
    物质类
    Chemical
    背景
    Iododeoxyuridine (5-Iodo-2'-deoxyuridine, IdU) is a synthetic thymidine nucleoside analog. IdU, like BrdU thymidine analog, is used to allow the detection of growing or proliferating cells in living tissues. During the S-phase of cell division, DNA replication occurs, and IdU can be incorporated into the newly synthesized DNA by substituting for naturally occurring thymidine. Antibodies specific for IdU are subsequently used to detect the incorporated IdU thymidine analog. This highlights cells that were actively replicating their DNA and is suggestive of actively growing cells. Antibody binding usually requires the DNA to be denatured, typically by exposing the cells to acid or heat. Synonyms: Iododeoxyuridine, 5-iodo-2'-deoxyuridine, IdU
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