BrdU 抗体
(2 references)-
- 抗原 See all BrdU 抗体
- BrdU (Bromodeoxyuridine (BrdU))
- 适用
- 请咨询
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This BrdU antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), ELISA, Fluorescence Microscopy (FM), Dot Blot (DB)
- 原理
- BrdU Antibody
- 交叉反应 （详细）
- This antibody reacts strongly with BrdU.
- 产品特性
- Synonyms: mouse anti-BrdU Antibody, Bromodeoxyuridine, 5-bromo-2'-deoxyuridine, BrdU
- 纯化方法
- Anti-BrdU Monoclonal Antibody was purified from ascites fluid by Protein A chromatography.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: Anti-BrdU monoclonal antibody was produced in mice by repeated immunizations prepared via immunizations with BromodeoxyUridine-KLH followed by hybridoma development.
Immunogen Type: Other
- 克隆位点
- 29G6-E8
- 亚型
- IgG1 lambda
- Top Product
- Discover our top product BrdU Primary Antibody
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anti-Bromodeoxyuridine (BrdU) antibody
BrdU FACS, IHC (p), ICC, IHC (fro) 宿主: 小鼠 Monoclonal Bu20a unconjugated
anti-Bromodeoxyuridine (BrdU) antibodyBrdU IHC (p), IF (cc), IF (p), IHC (fro), WB 宿主: 小鼠 Monoclonal A7 unconjugated
anti-Bromodeoxyuridine (BrdU) antibodyBrdU IHC (p), IF (cc), IF (p), IHC (fro), ELISA 宿主: 兔 Polyclonal unconjugated
anti-Bromodeoxyuridine (BrdU) antibodyBrdU IHC, Neut, StM 宿主: 小鼠 Monoclonal BRD469 unconjugated
anti-Bromodeoxyuridine (BrdU) antibodyBrdU IHC (p), IF (cc), IF (p), ICC, IHC (fro), ELISA 宿主: 兔 Polyclonal unconjugated
anti-Bromodeoxyuridine (BrdU) antibodyBrdU 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 小鼠 Monoclonal 4B6C12 unconjugated
anti-Bromodeoxyuridine (BrdU) antibodyBrdU IHC, Neut, StM 宿主: 兔 Monoclonal BRD2888R unconjugated Recombinant Antibody
anti-Bromodeoxyuridine (BrdU) antibody (PE)BrdU FACS 宿主: 小鼠 Monoclonal Bu20a PE
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- 应用备注
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Flow Cytometry Dilution: 1:50-1:100
Immunohistochemistry Dilution: 1:100-1:500
Application Note: Anti-BrdU Antibody has been tested as suitable for immunofluorescence and immunoblot assays. Antibody may be suitable for additional immunoassays including flow cytometry and immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Antibody will detect incorporated BrdU thymidine analog from replicated cells.
Western Blot Dilution: 1:2000 - 1:5000
ELISA Dilution: 1:2000 - 1:10000
IF Microscopy Dilution: 1:500-1:3000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store BrdU Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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: "Cell-Intrinsic Control of Interneuron Migration Drives Cortical Morphogenesis." in: Cell, Vol. 172, Issue 5, pp. 1063-1078.e19, (2019) (PubMed).
: "Parvovirus Expresses a Small Noncoding RNA That Plays an Essential Role in Virus Replication." in: Journal of virology, Vol. 91, Issue 8, (2017) (PubMed).
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: "Cell-Intrinsic Control of Interneuron Migration Drives Cortical Morphogenesis." in: Cell, Vol. 172, Issue 5, pp. 1063-1078.e19, (2019) (PubMed).
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- 抗原
- BrdU (Bromodeoxyuridine (BrdU))
- 别名
- BrdU (BrdU 产品)
- 物质类
- Chemical
- 背景
- Background: Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU) is a synthetic thymidine nucleoside analog. BrdU is commonly used to allow the detection of growing or proliferating cells in living tissues. During the S-phase of cell division, DNA replication occurs, and BrdU can be incorporated into the newly synthesized DNA by substituting for naturally occurring thymidine. Antibodies specific for BrdU are subsequently used to detect the incorporated BrdU thymidine analog. This highlights cells that were actively replicating their DNA and is suggestive of actively growing cells. Antibody binding usually requires the DNA to be denatured, typically by exposing the cells to acid or heat.
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