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RFP 抗体

Name: RFP 抗体
SKU: ABIN1607680
Availability: OutOfStock

Rockland 200-301-379 RFP 适用: Discosoma WB, ELISA, IP, FACS, FM 宿主: 小鼠 Monoclonal 8E5-G7 unconjugated

Western Blot of Mouse Anti-RFP Antibody Western Blot of Mouse Anti-RFP Antibody. (RFP 抗体)

Characterization of the transgenic model and effect of 4D on the RMSAFluorescence image of a sagittal section of a 4D+ brain after a 4-day treatment with doxycycline showing RFP signal confined to the SVZ and RMS (nuclei counterstained with DAPI, blue). (RFP 抗体)

IF (p)

ELISA

Western Blot of Mouse Anti-RFP antibody. (RFP 抗体)

Transgenic model and effects of acute 4D overexpression on NSC and progenitors of the SVZDrawings of the nestinCreERT2 (Imayoshi et al, 2008), ROSA26rtTA-flox (Belteki et al, 2005), and tet4D-RFP (Nonaka-Kinoshita et al, 2013) alleles of the 4D line. (RFP 抗体)

IF (p)

Chronic effect of 4D overexpression on NSC and OB neurogenesisAExperimental design used to assess the chronic effect of a transient 4D induction with BrdU and EdU given during Dox administration or 1 h before sacrifice, respectively. (RFP 抗体)

IF (p)

9 images

(16 references)

产品编号 ABIN1607680

Supplier Product No.: 200-301-379

发货至: 中国

Datasheet as PDF

抗原 See all RFP 抗体

RFP (Red Fluorescent Protein (RFP))

适用

Discosoma
宿主
22

12

9

5

3
小鼠
Compatible Secondaries
克隆类型
29

22
单克隆
标记
17

5

3

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This RFP antibody is un-conjugated
应用范围
25

19

14

11

10

8

7

6

3

2

2

1

1
Western Blotting (WB), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Supplier Product No.

200-301-379

Supplier

Rockland

原理

RFP Antibody

交叉反应（详细）

Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry.

产品特性

Synonyms: mouse anti-RFP Antibody, DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583

纯化方法

Anti-RFP Monoclonal Antibody was purified from concentrated tissue culture supernate by Protein A chromatography.

过滤

Sterile filtered

免疫原

Immunogen: The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full-length amino acid sequence (234aa) derived from the mushroom anemone Discosoma.
Immunogen Type: Recombinant Protein

克隆位点

8E5-G7

亚型

IgG2a kappa

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anti-Red Fluorescent Protein (RFP) (AA 21-245) antibody
RFP 适用: Discosoma WB, ELISA 宿主: 小鼠 Monoclonal unconjugated

应用备注

Flow Cytometry Dilution: User Optimized
Application Note: Monoclonal anti-RFP is designed to detect RFP and its variants. This antibody has been tested by ELISA and Western blot, and is suitable for use in FISH, IF, IHC, and multiplex assays based on published references. This antibody can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen. Optimal titers for applications should be determined by the researcher.
Western Blot Dilution: 1:1,000 - 1:10,000
Immunoprecipitation Dilution: User Optimized
ELISA Dilution: 1:75,000 - 1:150,000
IF Microscopy Dilution: User Optimized

限制

仅限研究用
状态

Liquid

浓度

1 mg/mL

缓冲液

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store anti-RFP at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

有效期

12 months
Singh, Elenio, Leu, Romano, Vaughan, DeRiso, Surendran, Chakrabarti: "A new Elf5CreERT2-GFP BAC transgenic mouse model for tracing Elf5 cell lineages in adult tissues." in: FEBS letters, Vol. 593, Issue 10, pp. 1030-1039, (2020) (PubMed).

Guan, Quiñones-Frías, Akbergenova, Littleton: "Drosophila Synaptotagmin 7 negatively regulates synaptic vesicle release and replenishment in a dosage-dependent manner." in: eLife, Vol. 9, (2020) (PubMed).

Donovan, Spencer, Kitt, Eastman, Lobur, Jiao, Silver, Deneris: "Lmx1b is required at multiple stages to build expansive serotonergic axon architectures." in: eLife, Vol. 8, (2020) (PubMed).

Winkowski, Nagode, Donaldson, Yin, Shamma, Fritz, Kanold: "Orbitofrontal Cortex Neurons Respond to Sound and Activate Primary Auditory Cortex Neurons." in: Cerebral cortex (New York, N.Y. : 1991), Vol. 28, Issue 3, pp. 868-879, (2019) (PubMed).

Katrancha, Shaw, Zhao, Myers, Cocco, Jeng, Zhu, Pittenger, Greer, Carr, Xiao, Koleske: "Trio Haploinsufficiency Causes Neurodevelopmental Disease-Associated Deficits." in: Cell reports, Vol. 26, Issue 10, pp. 2805-2817.e9, (2019) (PubMed).

Nicholson, Roberts, Sober: "Thalamostriatal and cerebellothalamic pathways in a songbird, the Bengalese finch." in: The Journal of comparative neurology, Vol. 526, Issue 9, pp. 1550-1570, (2019) (PubMed).

Bragado Alonso, Reinert, Marichal, Massalini, Berninger, Kuner, Calegari: "An increase in neural stem cells and olfactory bulb adult neurogenesis improves discrimination of highly similar odorants." in: The EMBO journal, Vol. 38, Issue 6, (2019) (PubMed).

Zhou, Rickmann, Hafner, Staiger: "Subcellular Targeting of VIP Boutons in Mouse Barrel Cortex is Layer-Dependent and not Restricted to Interneurons." in: Cerebral cortex (New York, N.Y. : 1991), Vol. 27, Issue 11, pp. 5353-5368, (2018) (PubMed).

Rider, Cheerathodi, Hurwitz, Nkosi, Howell, Tremblay, Liu, Zhu, Meckes: "The interactome of EBV LMP1 evaluated by proximity-based BioID approach." in: Virology, Vol. 516, pp. 55-70, (2018) (PubMed).

Guan, Cao, Liu, Zhao, Fu, Yu: "Eye opening differentially modulates inhibitory synaptic transmission in the developing visual cortex." in: eLife, Vol. 6, (2018) (PubMed).

Hou, Zhang: "NMDA Receptors Regulate the Development of Neuronal Intrinsic Excitability through Cell-Autonomous Mechanisms." in: Frontiers in cellular neuroscience, Vol. 11, pp. 353, (2017) (PubMed).

Bifari, Decimo, Pino, Llorens-Bobadilla, Zhao, Lange, Panuccio, Boeckx, Thienpont, Vinckier, Wyns, Bouché, Lambrechts, Giugliano, Dewerchin, Martin-Villalba, Carmeliet: "Neurogenic Radial Glia-like Cells in Meninges Migrate and Differentiate into Functionally Integrated Neurons in the Neonatal Cortex." in: Cell stem cell, Vol. 20, Issue 3, pp. 360-373.e7, (2017) (PubMed).

Meese, Cepeda, Gahlen, Adams, Ficner, Ricci, Heller, Reisinger, Herget: "Activity-Dependent Phosphorylation by CaMKIIδ Alters the Ca2+ Affinity of the Multi-C2-Domain Protein Otoferlin." in: Frontiers in synaptic neuroscience, Vol. 9, pp. 13, (2017) (PubMed).

Clarkson, Liptak, Gee, Sohal, Bender: "D3 Receptors Regulate Excitability in a Unique Class of Prefrontal Pyramidal Cells." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 37, Issue 24, pp. 5846-5860, (2017) (PubMed).

Wu, Kim, Seravalli, Barycki, Hart, Gohara, Di Cera, Jung, Kosman, Lee: "Potassium and the K+/H+ Exchanger Kha1p Promote Binding of Copper to ApoFet3p Multi-copper Ferroxidase." in: The Journal of biological chemistry, Vol. 291, Issue 18, pp. 9796-806, (2016) (PubMed).

Alexandre, Ghilas, Sanchez, Le Bon, Crozat, Dalod: "XCR1+ dendritic cells promote memory CD8+ T cell recall upon secondary infections with Listeria monocytogenes or certain viruses." in: The Journal of experimental medicine, Vol. 213, Issue 1, pp. 75-92, (2016) (PubMed).
抗原

RFP (Red Fluorescent Protein (RFP))

别名

DsRed (RFP 产品)

背景

Background: Antibodies to RFP (Discosoma spp.) are intended for use in immunological assays including ELISA, Western blotting, immunohistochemistry, and flow cytometry. RFP Proteins are useful markers for imagining protein localization, monitoring physiological processes, and detecting transgenic expression. Rockland's anti-RFP antibody can be used to detect native RFP and RFP variants.

UniProt

Q9U6Y8