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- 抗原 See all Pyruvate Kinase (PK) 抗体
- Pyruvate Kinase (PK)
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适用
- 兔
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This Pyruvate Kinase antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), ELISA
- 交叉反应 （详细）
- Cross reactivity against Pyruvate Kinase from other sources may occur but have not been specifically determined.
- 纯度
- Anti-Pyruvate Kinase Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Pyruvate Kinase [Rabbit Muscle]. Anti-Pyruvate Kinase has been reported to react with all forms of pyruvate kinase (pan M-PK).
- 内毒素水平
- Low Endotoxin : No
- 免疫原
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Pyruvate Kinase [Rabbit Muscle]
Immunogen Type: Native Protein - 亚型
- IgG
- Top Product
- Discover our top product PK Primary Antibody
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anti-Pyruvate Kinase (PK) (Center) antibody
PK 适用: 人, 大鼠 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Pyruvate Kinase (PK) (AA 476-505), (C-Term) antibodyPK 适用: 人, 小鼠, 大鼠, 猴 WB, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Pyruvate Kinase (PK) antibodyPK 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, IF/ICC 宿主: 兔 Polyclonal unconjugated
anti-Pyruvate Kinase (PK) (AA 121-151), (N-Term) antibodyPK 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Pyruvate Kinase (PK) (AA 476-505), (C-Term) antibodyPK 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Anti-Pyruvate Kinase Antibody has been assayed against 1.0 µg of Pyruvate Kinase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:3.000 to 1:15.000 of the reconstitution concentration is suggested for Anti-Pyruvate Kinase Antibody.
ELISA Dilution: 1:4.000 - 1:20.000
Western Blot Dilution: 1:500 - 1:2.000 - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 10 mg/mL
- 缓冲液
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0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free - 注意事项
- Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
- 有效期
- Expiration date is one (1) year from date of opening.
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- 抗原
- Pyruvate Kinase (PK)
- 别名
- Pyruvate Kinase (PK 产品)
- 别名
- CG7070 antibody, Dmel\\CG7070 antibody, PYK antibody, Pyk antibody, PYK3b antibody, PYK3c antibody, Pyruvate kinase antibody, PK antibody, pyruvate kinase antibody, pyruvate kinase (PK) antibody, Pyruvate kinase (PK) antibody, pyruvate kinase PKM antibody, testis expressed 45 antibody, PyK antibody, pykA antibody, PYK3a|PYK3b|PYK3c antibody, pyk antibody, pyk1 antibody, pk antibody, LOC100534543 antibody, TEX45 antibody
- 背景
- Pyruvate Kinase is an enzyme that is key in the glycolytic process. Pyruvate Kinase's specific activity is to catalyze the transfer of a phosphate group from phosphoenolpyruvate to ADP, yielding one molecule of pyruvate and one molecule of ATP. A isoenzyme of Pyruvate Kinase, known as the M2, is a key area of study in tumorigenesis, as the M2 isoenzyme seems to elevate aerobic glycolysis in tumor cells, leading to tumor proliferation. Conflicting evidence as to the importance of this isoenzyme in tumor growth establishes this as a large area of explporation and study. Anti-Pyurvate Kinase Antibody is ideal for investigators in Cancer, Cell Biology, and Signal Transduction research. Synonyms: Pyruvate kinase isozymes M1/M2 antibody, Pyruvate kinase muscle isozyme antibody, Pyruvate kinase 2/3 antibody, Cytosolic thyroid hormone-binding protein antibody
- 基因ID
- 100008676
- NCBI登录号
- NP_001182574
- UniProt
- P11974
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