Lactate Dehydrogenase A 抗体 (Biotin)
(1 reference)
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- 抗原 See all Lactate Dehydrogenase A (LDHA) 抗体
- Lactate Dehydrogenase A (LDHA)
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适用
- 兔
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This Lactate Dehydrogenase A antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP)
- Supplier Product No.
- 200-1673-0100
- Supplier
- Rockland
- 原理
- Lactate Dehydrogenase Antibody Biotin Conjugated
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Lactate Dehydrogenase [Rabbit Muscle].
- 产品特性
- Synonyms: goat anti-Lactate Dehydrogenase Antibody biotin Conjugation, biotin Conjugated goat anti-Lactate Dehydrogenase Antibody, L-lactate dehydrogenase A chain, LDH-A, LDH muscle subunit, LDH-M
- 纯化方法
- Anti-lactate dehydrogenase antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- 免疫原
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Immunogen: Lactate Dehydrogenase [full length protein isolated from rabbit muscle]
Immunogen Type: Native Protein
- 亚型
- IgG
- Top Product
- Discover our top product LDHA Primary Antibody
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anti-Lactate Dehydrogenase A (LDHA) antibody
LDHA 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Lactate Dehydrogenase A (LDHA) (AA 1-332) antibodyLDHA 适用: Pig WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Lactate Dehydrogenase A (LDHA) antibodyLDHA 适用: 人 WB, IHC, IP, IHC (p) 宿主: 小鼠 Monoclonal 2D11 unconjugated
anti-Lactate Dehydrogenase A (LDHA) (AA 1-332) antibodyLDHA 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Lactate Dehydrogenase A (LDHA) (AA 1-332) antibodyLDHA 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Lactate Dehydrogenase A (LDHA) (Middle Region) antibodyLDHA 适用: 人, 小鼠, 大鼠, 兔, Cow, 马, 豚鼠, 犬, 山羊, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Lactate Dehydrogenase A (LDHA) (AA 204-232) antibodyLDHA 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Lactate Dehydrogenase A (LDHA) (AA 154-182) antibodyLDHA 适用: 人 WB, ELISA, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Lactate Dehydrogenase A (LDHA) (AA 5-323) antibodyLDHA 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Lactate Dehydrogenase A (LDHA) antibodyLDHA 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Application Note: Anti-Lactate Dehydrogenase Biotin antibody has been tested by ELISA, Western blot, and immunofluorescence. This antibody is assayed against 1.0 μg of Lactate Dehydrogenase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:2,500
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:2,500 - 1:6,500
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 10 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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: "Intracellular mGluR5 plays a critical role in neuropathic pain." in: Nature communications, Vol. 7, pp. 10604, (2016) (PubMed).
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: "Intracellular mGluR5 plays a critical role in neuropathic pain." in: Nature communications, Vol. 7, pp. 10604, (2016) (PubMed).
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- 抗原
- Lactate Dehydrogenase A (LDHA)
- 别名
- LDHA (LDHA 产品)
- 背景
- Background: Anti-lactate dehydrogenase recognizes lactate dehydrogenase which catalyzes the interconversion of pyruvate to lactate and NADH to NAD+. This reaction occurs in the absence or lack of oxygen, making lactate dehydrogenase critical for anaerobic respiration. There five known isozymes of LDH, each localized in different types of tissue. In medicine, LDH is often used as a marker of various diseases ranging from hemolysis to encephalitis. Anti-lactate dehydrogenase antibody is ideal for investigators interested in Metabolism, Cancer, and Signal Transduction.
- 基因ID
- 100009107, 126050
- UniProt
- P13491
- 途径
- Warburg Effect
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