Lactate Dehydrogenase 抗体 (Biotin)
LDH
适用: 兔
WB, ELISA, IP
宿主: 山羊
Polyclonal
Biotin
(1 reference)-
- 抗原 See all Lactate Dehydrogenase (LDH) 抗体
- Lactate Dehydrogenase (LDH)
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适用
- 兔
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This Lactate Dehydrogenase antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), ELISA, Immunoprecipitation (IP)
- 交叉反应 （详细）
- Cross reactivity against Lactate Dehydrogenase from other sources may occur but have not been specifically determined.
- 纯度
- Anti-lactate dehydrogenase antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Lactate Dehydrogenase [Rabbit Muscle].
- 内毒素水平
- Low Endotoxin : No
- 免疫原
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Lactate Dehydrogenase [full length protein isolated from rabbit muscle]
Immunogen Type: Native Protein - 亚型
- IgG
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anti-Lactate Dehydrogenase (LDH) antibody (HRP)
LDH 适用: 兔 WB, ELISA 宿主: 山羊 Polyclonal HRP
anti-Lactate Dehydrogenase (LDH) antibodyLDH 适用: 兔 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
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- 应用备注
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Anti-lactate dehydrogenase antibody has been assayed against 1.0 µg of Lactate Dehydrogenase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4.000 to 1:20.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:20.000
IF Immunoprecipitation Dilution: 1:100
Western Blot Dilution: 1:500 - 1:5.000 - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 10 mg/mL
- 缓冲液
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0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free - 注意事项
- Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
- 有效期
- Expiration date is one (1) year from date of opening.
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: "Intracellular mGluR5 plays a critical role in neuropathic pain." in: Nature communications, Vol. 7, pp. 10604, (2016) (PubMed).
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: "Intracellular mGluR5 plays a critical role in neuropathic pain." in: Nature communications, Vol. 7, pp. 10604, (2016) (PubMed).
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- 抗原
- Lactate Dehydrogenase (LDH)
- 别名
- Lactate Dehydrogenase (LDH 产品)
- 别名
- CG10160 antibody, Dmel\\CG10160 antibody, IMP-L3 antibody, IMPL3 antibody, Imp-L3 antibody, Impl3 antibody, LDH antibody, Ld antibody, Ldh antibody, dLdha antibody, dmLDH antibody, Ecdysone-inducible gene L3 antibody, lactate dehydrogenase antibody, ldh2 antibody, ImpL3 antibody, ldh antibody, ldh2 antibody, Ldh antibody, LDH-A antibody
- 背景
- Anti-lactate dehydrogenase recognizes lactate dehydrogenase which catalyzes the interconversion of pyruvate to lactate and NADH to NAD+. This reaction occurs in the absence or lack of oxygen, making lactate dehydrogenase critical for anerobic respiration. There five known isozymes of LDH, each localized in different types of tissue. In medicine, LDH is often used as a marker of various diseases ranging from hemolysis to encephalitis. Anti-lactate dehydrogenase antibody is ideal for investigators interested in Metabolism, Cancer, and Signal Transduction. Synonyms: L-lactate dehydrogenase A chain LDH-A LDH muscle subunit LDH-M
- 基因ID
- 100009107, 126050
- UniProt
- P13491
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