Aldolase 抗体 (Biotin)
-
- 抗原 See all Aldolase (ALD) 抗体
- Aldolase (ALD)
-
适用
- 人, 兔
-
宿主
- 山羊
-
克隆类型
- 多克隆
-
标记
- This Aldolase antibody is conjugated to Biotin
-
应用范围
- Western Blotting (WB), ELISA, Immunoprecipitation (IP)
- 交叉反应 (详细)
- Cross reactivity against Aldolase from other sources may occur but have not been specifically determined.
- 纯度
- Anti-Aldolase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Aldolase [Rabbit Muscle].
- 内毒素水平
- Low Endotoxin : No
- 免疫原
-
Aldolase [Rabbit Muscle]
Immunogen Type: Native Protein - 亚型
- IgG
- Top Product
- Discover our top product ALD Primary Antibody
-
-
- 应用备注
-
Anti-Aldolase has been assayed against 1.0 µg of Aldolase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4.000 to 1:16.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:20.000
IF Immunoprecipitation Dilution: 1:100
Western Blot Dilution: 1:500 - 1:5.000 - 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 10 mg/mL
- 缓冲液
-
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free - 注意事项
- Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
- 有效期
- Expiration date is one (1) year from date of opening.
-
- 抗原
- Aldolase (ALD)
- 别名
- Aldolase (ALD 产品)
- 别名
- ALD antibody, BcDNA:LP07735 antibody, CG6058 antibody, Dmel\\CG6058 antibody, FBA antibody, T12 antibody, T4 antibody, T6 antibody, T7 antibody, ald antibody, Aldolase antibody, Ald antibody
- 背景
- Part of the class I fructose-bisphosphate aldolase family, the Anti-Aldolase antibody is essential in the processes glycolysis and gluconeogenesis, as well as performing the role of a scaffolding protein. Anti-Aldolase antibody is ideal for investigators interested in Metabolism, Cancer, and Signal Transduction research. Synonyms: Fructose-bisphosphate aldolase A Muscle-type aldolase
- 基因ID
- 100009055
- UniProt
- P00883
-