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Pyranose Oxidase 抗体 (Biotin)

适用: Microbial IP, WB, ELISA 宿主: 山羊 Polyclonal Biotin
产品编号 ABIN1607646
发货至: 中国
  • 抗原 See all Pyranose Oxidase products
    Pyranose Oxidase
    适用
    Microbial
    宿主
    • 3
    山羊
    克隆类型
    • 3
    多克隆
    标记
    • 2
    • 1
    This Pyranose Oxidase antibody is conjugated to Biotin
    应用范围
    Immunoprecipitation (IP), Western Blotting (WB), ELISA
    交叉反应 (详细)
    Cross reactivity against Pyranose Oxidase from other sources is unknown.
    纯度
    Anti-Pyranose oxidase antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Pyranose Oxidase [E.coli].
    内毒素水平
    Low Endotoxin : No
    免疫原
    Pyranose Oxidase [E.coli]
    Immunogen Type: Native Protein
    亚型
    IgG
  • 应用备注
    Anti-Pyranose oxidase antibody has been assayed against 1.0 µg of Pyranose Oxidase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:70.000 to 1:350.000 of the reconstitution concentration is suggested for this product.
    ELISA Dilution: 1:5.000 - 1:20.000
    IF Immunoprecipitation Dilution: 1:100
    Western Blot Dilution: 1:500 - 1:5.000
    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    10 mg/mL
    缓冲液
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
    注意事项
    Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
    储存条件
    -20 °C
    储存方法
    Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
    有效期
    Expiration date is one (1) year from date of opening.
  • 抗原
    Pyranose Oxidase
    Abstract
    Pyranose Oxidase 产品
    别名
    Pyranose oxidase antibody, pyranose oxidase antibody, Achl_3529 antibody, Kvar_1112 antibody, AMED_5392 antibody
    背景
    Anti-Pyranose oxidase recognizes the oxidoreductase pyranose oxidase. In general, pyranose catalyzes the oxidation of aldopyranoses at the carbon 2 position to form 2-ketoaldoses . Notably, pyranose oxidase catalyzes the conversion of D-glucose and oxygen to 2-dehydro-D-glucose and hydrogen peroxide using flavin adenine dinucleotide (FAD) as a cofactor. Pyranose oxidase also plays a role in the pentose phosphate pathway. Synonyms: P2Ox Pyranose oxidase PROD POD POx EC=1.1.3.10 Pyranose:oxygen 2-oxidoreductase Glucose 2-oxidase FAD-oxidoreductase
    UniProt
    Q5G234, P79076
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