ARFGAP3 抗体
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- 抗原 See all ARFGAP3 抗体
- ARFGAP3 (ADP-Ribosylation Factor GTPase Activating Protein 3 (ARFGAP3))
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This ARFGAP3 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Fluorescence Microscopy (FM)
- Supplier Product No.
- 100-401-h46
- Supplier
- Rockland
- 原理
- ARFGAP3 Antibody
- 交叉反应 （详细）
- Further purification was used to remove the GST tag.
- 产品特性
- Synonyms: rabbit anti-ArfGAP3 Antibody, ARFGAP1, ADP-ribosylation factor GTPase-activating protein 3, ARF GAP3, Arf-GAP, ArfGAP
- 纯化方法
- Anti-ArfGAP3 antibody was prepared from monospecific antiserum by delipidation and defibrination.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: This whole rabbit serum was prepared by repeated immunizations with a truncated recombinant sequence of ArfGAP3 fused to GST.
Immunogen Type: Recombinant Protein
- Top Product
- Discover our top product ARFGAP3 Primary Antibody
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anti-ADP-Ribosylation Factor GTPase Activating Protein 3 (ARFGAP3) (AA 217-516) antibody
ARFGAP3 适用: 人 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-ADP-Ribosylation Factor GTPase Activating Protein 3 (ARFGAP3) (C-Term) antibodyARFGAP3 适用: 人, 小鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-ADP-Ribosylation Factor GTPase Activating Protein 3 (ARFGAP3) (C-Term) antibodyVerified ARFGAP3 适用: 小鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-ADP-Ribosylation Factor GTPase Activating Protein 3 (ARFGAP3) (AA 377-426) antibodyARFGAP3 适用: 人, 犬 WB 宿主: 兔 Polyclonal unconjugated
anti-ADP-Ribosylation Factor GTPase Activating Protein 3 (ARFGAP3) (AA 91-140) antibodyARFGAP3 适用: 人, 豚鼠, 马, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Application Note: ArfGAP3 has been tested for use in Immunofluorescence and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 57 kDa in size by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:1,000
Immunoprecipitation Dilution: User Optimized
ELISA Dilution: 1:5,000
IF Microscopy Dilution: 1:100
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Preservative: 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- ARFGAP3 (ADP-Ribosylation Factor GTPase Activating Protein 3 (ARFGAP3))
- 别名
- ARFGAP3 (ARFGAP3 产品)
- 背景
- Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. The ArfGAPs are a family of proteins encoded by 31 genes in humans. The function of the ArfGAPs is to regulate the small G protein Arf, a regulator of membrane traffic and actin cytoskeleton. The ArfGAPs have been found to be key regulators of cellular behaviors that involve coordinated actin and membrane remodeling, including protein secretion and migration. Several ArfGAPs are associated with cancer cell invasion and metastasis, and the Arf pathway has been found to be affected in a number of genetic diseases. Although the importance of the ArfGAPs and the Arf pathway for cellular physiology is well recognized, the molecular basis for the function of these proteins has not been established. Reagents for the studies, especially antibodies specific for particular members of the ArfGAP family that can be used for immunoblotting and immunofluorescence, are not available. ArfGAP3 is one of three ArfGAPs thought to be specifically associated with the Golgi apparatus, however, localization has only been done for ectopically expressed recombinant protein and cellular function is still not established. Indeed, there is a growing controversy about the function of ArfGAP3 together with ArfGAP1 and ArfGAP2. Antibodies suitable for immunoprecipitation, immunoblotting and immunofluorescence would be of great value in addressing the controversy and for discovering the potential role of ArfGAP3 in secretion of proteins, such as growth factors, and trafficking of other proteins to the cell surface, such as growth factor receptors and cell adhesion molecules.
- 基因ID
- 26286
- NCBI登录号
- NP_001135765
- UniProt
- Q9NP61
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