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Patched 1 抗体 (AA 266-280)

Verified PTCH1 适用: 人, 小鼠 WB, ELISA, IHC, IF 宿主: 山羊 Polyclonal unconjugated
产品编号 ABIN1590007
发货至: 中国
  • 抗原 See all Patched 1 (PTCH1) 抗体
    Patched 1 (PTCH1)
    抗原表位
    • 5
    • 5
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 266-280
    适用
    • 38
    • 26
    • 9
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    人, 小鼠
    宿主
    • 35
    • 7
    • 3
    山羊
    克隆类型
    • 39
    • 6
    多克隆
    标记
    • 33
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Patched 1 antibody is un-conjugated
    应用范围
    • 31
    • 22
    • 16
    • 11
    • 6
    • 5
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)
    原理
    PTCH1 (aa266-280)
    序列
    EELKKINYQV DSWEE
    特异性
    This antibody is expected to recognize all reported isoforms (NP_000255.2, NP_001077072.1, NP_001077071.1, NP_001077073.1). Reported variants represent identical protein: NP_001077075.1, NP_001077074.1, NP_001077073.1, NP_001077076.1
    交叉反应
    Cow, 犬, 人, 小鼠, 大鼠
    纯化方法
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    质量等级
    Verified
    免疫原
    Peptide with sequence C-EELKKINYQVDSWEE, from the C or N Terminus of the protein sequence according to NP_000255.2, NP_001077072.1, NP_001077071.1, NP_001077073.1.
    亚型
    IgG
    Top Product
    Discover our top product PTCH1 Primary Antibody
  • 应用备注

    Immunohistochemistry: Paraffin embedded Human Brain (Cortex). Recommended concentration: 8 μg/mL.

    Western Blot: Approx 140 kDa band observed in Mouse fetal Kidney lysates (calculated MW of 159 kDa according to NP_032983.1). This molecular weight is routinely observed by other sources. Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour.

    Peptide ELISA: antibody detection limit dilution 1:32000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 5μg/ml.

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.5 mg/mL
    缓冲液
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Minimize freezing and thawing.
    储存条件
    -20 °C
    储存方法
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原
    Patched 1 (PTCH1)
    别名
    PTCH1 (PTCH1 产品)
    别名
    BCNS antibody, HPE7 antibody, NBCCS antibody, PTC antibody, PTC1 antibody, PTCH antibody, PTCH11 antibody, lep antibody, ptc1 antibody, ptc2 antibody, Ptch antibody, Ptch2 antibody, Ptc-1 antibody, XPtc1 antibody, patched antibody, patched1 antibody, A230106A15Rik antibody, Ptc antibody, Ptc1 antibody, mes antibody, patched 1 antibody, patched 1 L homeolog antibody, Protein patched homolog 1 antibody, PTCH1 antibody, ptch1 antibody, Ptch1 antibody, ptch1.L antibody, ptc-1 antibody
    背景
    PTCH1, patched 1, BCNS, FLJ26746, FLJ42602, HPE7, NBCCS, PTC, PTC1, PTCH, PTCH11, OTTHUMP00000021709, OTTHUMP00000021710, PTCH protein +12b, PTCH protein +4', PTCH protein -10, patched homolog 1, protein patched homolog 1
    基因ID
    5727, 19206, 89830
    NCBI登录号
    NP_000255, NP_001077072, NP_001077071, NP_001077073
    途径
    Hedgehog Signaling, Carbohydrate Homeostasis, Tube Formation
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