MAPKAP Kinase 2 抗体 (Internal Region)
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- 抗原 See all MAPKAP Kinase 2 (MAPKAPK2) 抗体
- MAPKAP Kinase 2 (MAPKAPK2) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2))
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抗原表位
- Internal Region
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适用
- 人, 小鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This MAPKAP Kinase 2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)
- 原理
- MK2 / MAPKAPK2
- 序列
- SRVLKEDKER WED
- 特异性
- This antibody is expected to recognize both reported isoforms (NP_004750.1, NP_116584.2).
- 交叉反应
- Cow, 犬, 人, 小鼠, 大鼠
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 亚型
- IgG
- Top Product
- Discover our top product MAPKAPK2 Primary Antibody
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- 应用备注
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Immunohistochemistry: Paraffin embedded Human Heart. Recommended concentration: 5 μg/mL.
Western Blot: Approx 60 kDa band observed in Mouse Embryonic Fibroblasts (MEF) lysates, not present in the knockout MEFs and reappearing as a 45 kDa band upon reintroduction of MK2 gene expression through viral transduction (calculated MW of 44.0 kDa accord
Peptide ELISA: antibody detection limit dilution 1:128000.
- 说明
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Immunoprecipitation: MK2/MK3 double knockout MEFs retrovirally transduced wiith MK2 or empty vector were lysed from confluent plates and used for IP with 1.5ug EB11929 or EB11930. Western blots of the IP were labelled with rabbit anti-MK2
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- 抗原
- MAPKAP Kinase 2 (MAPKAPK2) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2))
- 别名
- MAPKAPK2 (MAPKAPK2 产品)
- 别名
- mapkapk2 antibody, wu:fb30e10 antibody, wu:fc56c10 antibody, wu:fi03a01 antibody, MGC78852 antibody, MAPKAPK2 antibody, AA960234 antibody, MAPKAP-K2 antibody, MK-2 antibody, MK2 antibody, Rps6kc1 antibody, mitogen-activated protein kinase-activated protein kinase 2a antibody, mitogen-activated protein kinase-activated protein kinase 2 L homeolog antibody, mitogen-activated protein kinase-activated protein kinase 2 antibody, MAP kinase-activated protein kinase 2 antibody, mapkapk2a antibody, mapkapk2.L antibody, MAPKAPK2 antibody, Mapkapk2 antibody
- 背景
- MAPKAPK2, mitogen-activated protein kinase-activated protein kinase 2, MAPKAP-K2, MK-2, MK2, MAP kinase-activated protein kinase 2, MAPK-activated protein kinase 2, MAPKAP kinase 2
- 基因ID
- 9261, 17164, 289014
- NCBI登录号
- NP_004750, NP_116584
- 途径
- MAPK Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades
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