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KIT 抗体 (Internal Region)

Name: KIT 抗体 (Internal Region)
Brand: antibodies-online
SKU: ABIN1589992
Availability: OutOfStock

Verified KIT 适用: 人 WB, ELISA, FACS, IHC, IF 宿主: 山羊 Polyclonal unconjugated

ABIN1589992 (1 µg/mL) staining of Human Hippocampus lysate (35 µg protein in RIPA buffer). (KIT 抗体 (Internal Region))

ABIN1589992 (10µg/ml) staining of paraffin embedded Human Prostate. (KIT 抗体 (Internal Region))

IHC

ABIN1589992 Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0. (KIT 抗体 (Internal Region))

FACS

ABIN1589992 (1µg/ml) staining of Human Hippocampus lysate (A) + peptide (B) (35µg protein in RIPA buffer). (KIT 抗体 (Internal Region))

ABIN1589992 Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells, permeabilized with 0. (KIT 抗体 (Internal Region))

ABIN1589992 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. (KIT 抗体 (Internal Region))

6 images

产品编号 ABIN1589992

发货至: 中国

Datasheet as PDF

抗原 See all KIT 抗体

KIT (Mast/stem Cell Growth Factor Receptor (KIT))
抗原表位
55

54

34

33

23

23

23

19

16

16

16

16

16

16

14

13

13

12

12

11

10

8

8

8

8

8

8

7

7

7

7

7

7

7

7

7

7

7

7

7

7

7

7

7

7

7

6

6

6

6
Internal Region
适用
659

222

63

29

27

25

16

12

5

4

2

2

1
人
宿主
596

98

31

2
山羊
Compatible Secondaries
克隆类型
588

139

1
多克隆
标记
219

78

74

72

59

59

11

10

10

10

10

10

10

10

10

10

10

5

4

4

4

4

4

4

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1
This KIT antibody is un-conjugated
应用范围
415

385

279

147

91

91

61

47

44

42

30

24

10

6

6

6

2

2

1

1

1

1
Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)

原理

KIT / CD117

序列

KRDSFICSKQ EDH

特异性

This antibody is expected to recognize both reported isoforms (NP_000213.1, NP_001087241.1).

交叉反应

Cow, 犬, 人, Pig

纯化方法

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

质量等级

Verified

亚型

IgG

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KIT 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 2E3 unconjugated

anti-Mast/stem Cell Growth Factor Receptor (KIT) (AA 25-295) antibody
KIT 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Mast/stem Cell Growth Factor Receptor (KIT) antibody (FITC)
KIT 适用: 人, Cow, Non-Human Primate FACS 宿主: 小鼠 Monoclonal 104D2 FITC

anti-Mast/stem Cell Growth Factor Receptor (KIT) (AA 26-285) antibody
KIT 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Mast/stem Cell Growth Factor Receptor (KIT) (AA 106-135) antibody
KIT 适用: 人, 小鼠 WB, ELISA, FACS, IHC 宿主: 兔 Polyclonal unconjugated

应用备注

Immunohistochemistry: Paraffin embedded Human Prostate. Recommended concentration: 10 μg/mL.
Western Blot: Approx 110 kDa band observed in Human Brain (Hippocampus) lysates (calculated MW of 110 kDa according to NP_000213.1). An additional band of unknown identity was also consistently observed at 35 kDa. This band was successfully blocked by incub
Peptide ELISA: antibody detection limit dilution 1:128000.

说明

Immunofluorescence: Strong expression of the protein seen in the cytoplasm/ membranes of HEK293 and HeLa cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended co

限制

仅限研究用
状态

Liquid

浓度

0.5 mg/mL

缓冲液

Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Minimize freezing and thawing.

储存条件

-20 °C

储存方法

Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
抗原

KIT (Mast/stem Cell Growth Factor Receptor (KIT))

别名

KIT (KIT 产品)

别名

C-Kit antibody, CD117 antibody, PBT antibody, SCFR antibody, Bs antibody, Fdc antibody, Gsfsco1 antibody, Gsfsco5 antibody, Gsfsow3 antibody, SCO1 antibody, SCO5 antibody, SOW3 antibody, Ssm antibody, Tr-kit antibody, W antibody, c-KIT antibody, c-kit antibody, MGF antibody, KIT antibody, C-KIT antibody, kit antibody, receptor antibody, sparse antibody, Xkl-1 antibody, kit-a antibody, kit-b antibody, kl1-A antibody, krk1 antibody, pbt antibody, scfr antibody, KIT proto-oncogene receptor tyrosine kinase antibody, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog a antibody, KIT proto-oncogene receptor tyrosine kinase S homeolog antibody, KIT antibody, Kit antibody, kita antibody, kit antibody, kit.S antibody

背景

KIT, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit, CD117, PBT, SCFR, mast/stem cell growth factor receptor Kit, p145 c-kit, piebald trait protein, proto-oncogene c-Kit, proto-oncogene tyrosine-protein kinase Kit, soluble KIT varian

基因ID

3815

NCBI登录号

NP_000213, NP_001087241

途径

RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Sensory Perception of Sound, Stem Cell Maintenance, Production of Molecular Mediator of Immune Response, Regulation of long-term Neuronal Synaptic Plasticity