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- 抗原 See all Podoplanin (PDPN) 抗体
- Podoplanin (PDPN)
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Podoplanin antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC)
- 原理
- Podoplanin antibody
- 特异性
- Membrane protein fraction of isolated human glomeruli
- 产品特性
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Chromosomal location: 1p36.21
The monoclonal antibody was produced with the help of BALB/c mice using a membrane protein fraction of isolated human glomeruli as the immunizing antigen. - 纯化方法
- The mouse IgG1, kappa antibody (#4D5aE5E6) from hybridomas was purified from cell culture supernatant by Protein G chromatography.
- 免疫原
- Membrane protein fraction of isolated human glomeruli
- 克隆位点
- 4D5aE5E6
- 亚型
- IgG1, kappa
- Top Product
- Discover our top product PDPN Primary Antibody
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anti-Podoplanin (PDPN) (AA 91-166) antibody
PDPN 适用: 小鼠, 大鼠 WB, IHC (p), IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Podoplanin (PDPN) (AA 23-143) antibodyPDPN 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Podoplanin (PDPN) (AA 24-126) antibodyPDPN 适用: 人 ELISA, IF, IHC (f), Coat 宿主: 小鼠 Monoclonal PDPN-1433 unconjugated
anti-Podoplanin (PDPN) antibodyPDPN 适用: 小鼠 WB, FACS, IP, IHC (p) 宿主: 大鼠 Monoclonal 8F11 unconjugated
anti-Podoplanin (PDPN) antibodyPDPN 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Podoplanin (PDPN) antibodyPDPN 适用: 人, 小鼠 WB, ELISA 宿主: 小鼠 Monoclonal 5-00E-02 unconjugated
anti-Podoplanin (PDPN) antibodyPDPN 适用: 小鼠 WB, IP, IHC (fro) 宿主: Golden Syrian Hamster Monoclonal 811 unconjugated
anti-Podoplanin (PDPN) (AA 1-100) antibodyPDPN 适用: Synthetic ELISA, IF, IHC (f), Coat 宿主: 兔 Monoclonal unconjugated Recombinant Antibody
anti-Podoplanin (PDPN) (N-Term) antibodyPDPN 适用: 人, 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Podoplanin (PDPN) (AA 97-146) antibodyPDPN 适用: 人, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Immunohistochemistry: Cryo sections (acetone fixed), Paraffin sections (after autoclave or microwave treatment in 0.01 M citrate buffer, pH 6.0). Recommended working concentration is 0.5 μg/mL. WB: Use at 1-5 μg/mL
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.
- 缓冲液
- PBS
- 注意事项
- Centrifuge vial prior to opening.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.
- 有效期
- 24 months
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: "Heterogeneity of stromal cells in the human splenic white pulp. Fibroblastic reticulum cells, follicular dendritic cells and a third superficial stromal cell type." in: Immunology, Vol. 143, Issue 3, pp. 462-77, (2014) (PubMed).
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: "Heterogeneity of stromal cells in the human splenic white pulp. Fibroblastic reticulum cells, follicular dendritic cells and a third superficial stromal cell type." in: Immunology, Vol. 143, Issue 3, pp. 462-77, (2014) (PubMed).
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- 抗原
- Podoplanin (PDPN)
- 别名
- Podoplanin (PDPN 产品)
- 背景
- PDPN, T1A, GP36, GP40, Gp38, OTS8, T1A-2, AGGRUS, HT1A-1, PA2.26,Podoplanin, also known as glycoprotein 36 (gp36), PA2.26 antigen, T1alpha (T1A), and aggrus, is a 36 kDa type I transmembrane sialoglycoprotein and member of the Podoplanin family. Podoplanin has three potential splice variants, the longest of which is represented by a 238 amino acid (aa) precursor (NP_006465). It contains an undefined signal sequence, a 22 aa transmembrane segment (aa 207-228) and a short cytoplasmic tail (aa 229-238). The ECD contains abundant Ser/Thr residues that could serve as potential O-linked glycosolation sites. The cytoplasmic tail contains putative sites for protein kinase C phosphorylation. There are two potential alternate start sites at Met 77 (Swiss Prot #: Q86YL7) and Met 119 (EAW51692) that generate short forms. The 162 aa short form Podoplanin precursor shares 47 % aa identity with mouse Podoplanin. Podoplanin is expressed on glomerular epithelial cells (podocytes), type I lung alveolar cells, lymphatic endothelial cells, and numerous tumors, including colorectal tumors, squamous cell carcinomas, testicular seminoma, and brain tumors. One study shows high expression of Podoplanin mRNA in placenta, lung, skeletal muscle, and heart, and weaker levels in brain, kidney, and liver. Podoplanin is the ligand for C-type lectin-like receptor 2 (CLEC2). Their association is dependent on sialic acid on O-glycans of Podoplanin. Through its association with CLEC2, Podoplanin-induces platelet aggregation and tumor metastasis. Podoplanin is also necessary for lymphatic vessel formation, normal lung cell proliferation and alveolus formation at birth.
- 基因ID
- 10630
- NCBI登录号
- NM_001006624, NP_001006625
- UniProt
- Q86YL7
- 途径
- Dicarboxylic Acid Transport
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