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- 抗原 See all mCherry products
- mCherry (mCherry Fluorescent Protein)
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适用
- Discosoma
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This mCherry antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC)
- 纯化方法
- affinity purified antibody
- 克隆位点
- 1C51
- 亚型
- IgG2a
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anti-mCherry Fluorescent Protein antibody
适用: Discosoma WB, IF, IP, IHC (fro), IHC (p), IEM 宿主: 山羊 Polyclonal unconjugated
anti-mCherry Fluorescent Protein antibody适用: Anaplasma marginale WB, ICC, FACS 宿主: 兔 Polyclonal unconjugated
anti-mCherry Fluorescent Protein antibody适用: Discosoma WB 宿主: 兔 Polyclonal unconjugated
anti-mCherry Fluorescent Protein antibody适用: Discosoma WB 宿主: 小鼠 Polyclonal unconjugated
anti-mCherry Fluorescent Protein antibody适用: Discosoma WB 宿主: 小鼠 Monoclonal unconjugated
anti-mCherry Fluorescent Protein antibody适用: Discosoma WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-mCherry Fluorescent Protein antibody适用: Discosoma WB, IF, ICC 宿主: 小鸡 Polyclonal unconjugated
anti-mCherry Fluorescent Protein antibody适用: Discosoma WB, IP 宿主: 小鼠 Monoclonal unconjugated
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- 应用备注
- Try at dilutions of 1:500 and higher for immunofluorescence. For western blots try at 1:2,000.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store at 4°C short term or -20°C long term.
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- 抗原
- mCherry (mCherry Fluorescent Protein)
- 别名
- Cherry Dsred (mCherry 产品)
- 背景
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MCherry is derived from proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals), and is used as a fluorescent tracer in transfection and transgenic experiments. The prototype for these fluorescent proteins is Green Fluorescent Protein (GFP), which is a approx. 27 kDa protein isolated originally from the jellyfish Aequoria victoria. GFP was the basis of the 2008 Nobel Prize in Chemistry, awarded to Osamu Shimomura, Martin Chalfie and Roger Tsien, specifically “for the discovery and development of the green fluorescent protein, GFP“. GFP was shown to fluoresce on contact with molecular oxygen, requiring no other cofactors, and so can be expressed in fluorescent form in essentially any prokaryotic or eukaryotic cell.
The mCherry protein is derived from DsRed, a red fluorescent protein from so-called disc corals of the genus Discosoma. DsRed is similar in size and properties to GFP, but, obviously, produces a red rather than a green fluorochrome. The original DsRed was engineered extensively in the Tsien lab to prevent it from forming tetramers and dimers and to modify and improve the spectral properties. Several further cycles of mutation, directed modification and evolutionary selection produced mCherry, which has an excitation maximum at 587 nm and and emission maximum at 610 nm.
We expressed the mCherry protein sequence shown in some publications in bacteria, purified out the mCherry and raised a rabbit polyclonal antibody. This was affinity purified and was found to stain a band of the expected size in HEK293 cells transfected with a vector designed to express mCherry which was obtained from Clontech.
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