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- 抗原 See all GFP 抗体
- GFP (Green Fluorescent Protein (GFP))
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适用
- Aequorea victoria
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宿主
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小鸡
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克隆类型
- 多克隆
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标记
- This GFP antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunoprecipitation (IP)
- 特异性
- GFP Antibody, pAb, Chicken specifically reacts with fusion proteins containing GFP epitope tag. This antibody recognizes all variants of GFP protein such as eGFP, AcGFP1 and cGFP, and a variety of variants of RFP protein such as DsRed, DeRed2, mCherry and tdTomato. It slightly recognizes mOrange protein.
- 交叉反应 (详细)
- GFP Antibody, pAb, Chicken specifically reacts with fusion proteins containing GFP epitope tag. This antibody recognizes all variants of GFP protein such as eGFP, AcGFP1 and cGFP, and a variety of variants of RFP protein such as DsRed, DeRed2, mCherry and tdTomato. It slightly recognizes mOrange protein.
- 纯化方法
- Immunoaffinity chromatography
- 免疫原
- purified recombinant full-length GFP protein
- 亚型
- IgG2a
- Top Product
- Discover our top product GFP Primary Antibody
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- 应用备注
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Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.2-1 µg/mL Immunoprecipitation: 1-5 µg/mL Other Applications: user optimized - 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- PBS, pH 7.4, containing 0.02 % Sodium azide
- 储存液
- Sodium azide
- 注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
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Gene therapy rescues cilia defects and restores olfactory function in a mammalian ciliopathy model." in: Nature medicine, Vol. 18, Issue 9, pp. 1423-8, (2012) (PubMed).
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Gene therapy rescues cilia defects and restores olfactory function in a mammalian ciliopathy model." in: Nature medicine, Vol. 18, Issue 9, pp. 1423-8, (2012) (PubMed).
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- 抗原
- GFP (Green Fluorescent Protein (GFP))
- 别名
- GFP (GFP 产品)
- 别名
- green fluorescent protein antibody, gfp antibody
- 背景
- Green fluorescence protein (GFP) is a 27 kDa protein derived from jellyfish Aequorea victoria. It emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP has become a very useful tool as a fusion protein to report gene expression, trace cell lineage and define subcellular protein localizations. YFP differs from GFP due to a mutation at T203Y. GFP Antibody, pAb, Chicken is developed in chicken using purified recombinant full-length GFP protein.
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