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- 抗原 See all GAD65 (GAD2) 抗体
- GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))
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抗原表位
- N-Term
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This GAD65 antibody is un-conjugated
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应用范围
- Western Blotting (WB)
- 特异性
- Rabbit Anti-GAD65 Polyclonal Antibody detects endogenous levels of human GAD65.Positive Control: SH-SY5Y.
- 纯化方法
- Immunoaffinity chromatography
- 免疫原
- KLH-coupled synthetic peptide from N-terminal of human GAD65 .
- 亚型
- IgG1
- Top Product
- Discover our top product GAD2 Primary Antibody
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anti-Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) (AA 65-105) antibody
GAD2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) (AA 1-585) antibodyGAD2 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) (AA 188-374) antibodyGAD2 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) (AA 401-585) antibodyGAD2 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) (Internal Region) antibodyVerified GAD2 适用: 人 WB, IHC, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) (C-Term) antibodyGAD2 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) (AA 1-100) antibodyGAD2 适用: 人 WB, IHC, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 5D9G12 unconjugated
anti-Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) (AA 131-164), (N-Term) antibodyGAD2 适用: 人, 大鼠, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) (AA 109-138) antibodyGAD2 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB19330 unconjugated
anti-Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) (AA 501-585) antibodyGAD2 适用: 人, 大鼠, 小鸡 WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 1-2 µg/mLOther Applications: user-optimized - 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- PBS, pH 7.4, containing 0.02 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
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- 抗原
- GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))
- 别名
- GAD65 (GAD2 产品)
- 别名
- GAD65 antibody, gad65 antibody, F12P19.12 antibody, F12P19_12 antibody, GLUTAMATE DECARBOXYLASE 2 antibody, glutamate decarboxylase 2 antibody, zgc:112198 antibody, 6330404F12Rik antibody, GAD(65) antibody, Gad-2 antibody, glutamate decarboxylase 2 antibody, glutamate decarboxylase 1 antibody, glutamic acid decarboxylase 2 antibody, GAD2 antibody, PTRG_06148 antibody, LOC9324534 antibody, Gad2 antibody, GAD1 antibody, gad2 antibody
- 背景
- Glutamic Acid Decarboxylase (GAD) catalyzes the conversion of glutamate to gamma-aminobutyric acid (GABA), the principal inhibitory neurotransmitter in the brain. There are two isoforms of GAD, GAD65 (GAD2) and GAD67 (GAD1) (65 and 67 kDa, respectively) encoded by two different genes. With 64% amino acid identity, the two isoforms have distinct intraneuronal distribution in the brain. The highest degree of diversity appears to be located at the N-terminus. Primarily found in nerve terminals, GAD65 is required for targeting the enzyme to GABA-containing secretory vesicles. GAD67 is thought to be primarily located in the nerve cell body and has been identified as a major autoantigen in type 1 diabetes.Rabbit Anti-GAD65 Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide from N-terminal of human GAD65 (Swiss Prot: Q05329).
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