Rabbit Anti-Neurofascin Polyclonal Antibody detects endogenous levels of rat and mouse Neurofascin protein. It is predicted to react with human neurofascin protein according to sequence homology.
交叉反应 (详细)
Rabbit Anti-Neurofascin Polyclonal Antibody detects endogenous levels of rat and mouse Neurofascin protein. It is predicted to react with human neurofascin protein according to sequence homology.
纯化方法
Immunoaffinity chromatography
免疫原
KLH-coupled synthetic peptide from amino terminal of human Neurofascin .
NFASC
适用: 人, 大鼠, 小鼠
WB, IF (p), IF (cc)
宿主: 兔
Polyclonal
AbBy Fluor® 555
应用备注
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/mL Western blot: 1-2 µg/mLOther Applications: user-optimized
限制
仅限研究用
状态
Lyophilized
缓冲液
PBS, pH 7.4, containing 0.02 % sodium azide
储存液
Sodium azide
注意事项
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
储存条件
4 °C/-20 °C
储存方法
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Neurofascin is one member of Li family which involves vertebrate L1, Ng-CAM, and Nr-CAM. Neurofascin protein is a nervous system cell adhesion molecule that contains six Ig domains and multiple fibronectin type III repeats in its extracellular region. Expression level of neurofascin is abundant in the nervous system during early development as well as in adult stage. Phosphorylation of the FIGQY tyrosine of neurofascin abolishes ankyrin binding and increases lateral mobility of neurofascin. Phospho-FIGQY neurofascin associates with doublecortin, which probably plays an important role in neuronal migration.Rabbit Anti-Neurofascin Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide from amino terminal of human Neurofascin. (Swiss Prot: O94856).