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Geminin 抗体 (AA 50-100)

GMNN 适用: 人 WB, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN1574066
发货至: 中国
  • 抗原 See all Geminin (GMNN) 抗体
    Geminin (GMNN) (Geminin, DNA Replication Inhibitor (GMNN))
    抗原表位
    • 9
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    • 2
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    AA 50-100
    适用
    • 54
    • 11
    • 10
    宿主
    • 46
    • 10
    克隆类型
    • 44
    • 12
    多克隆
    标记
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    • 1
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    This Geminin antibody is un-conjugated
    应用范围
    • 31
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    • 12
    • 8
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    • 5
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    • 3
    • 2
    • 1
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    Western Blotting (WB), ELISA, Flow Cytometry (FACS)
    特异性
    Geminin Antibody, pAb, Rabbit detects endogenous levels of human geminin protein.
    纯化方法
    Immunoaffinity chromatography
    免疫原
    KLH-coupled synthetic peptide within AA 50-100 of human geminin protein .
    亚型
    IgG
    Top Product
    Discover our top product GMNN Primary Antibody
  • 应用备注
    Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

    ELISA: 0.05-0.2 µg/mL
    Western blot: 0.5-1 µg/mLFlow cytometry: 1-3 µg for 1 x 106 cellsOther applications: user-optimized
    限制
    仅限研究用
  • 状态
    Lyophilized
    缓冲液
    PBS, pH 7.4, containing 0.02 % sodium azide
    储存液
    Sodium azide
    注意事项
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    储存条件
    4 °C/-20 °C
    储存方法
    The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
  • Wang, Kirschner: "Emi1 preferentially inhibits ubiquitin chain elongation by the anaphase-promoting complex." in: Nature cell biology, Vol. 15, Issue 7, pp. 797-806, (2013) (PubMed).

  • 抗原
    Geminin (GMNN) (Geminin, DNA Replication Inhibitor (GMNN))
    别名
    Geminin (GMNN 产品)
    别名
    CG3183 antibody, Dm Geminin antibody, Dm geminin antibody, Dmel\\CG3183 antibody, Gem antibody, Geminin antibody, anon-WO0257455.1 antibody, gem antibody, l(2)k14019 antibody, v(2)k09107 antibody, sb:cb254 antibody, zgc:66217 antibody, GB16871 antibody, GMNN antibody, geminin antibody, dyak_GLEANR_833 antibody, DyakGE24654 antibody, GE24654 antibody, LOC100049517 antibody, AW546347 antibody, geminin, DNA replication inhibitor antibody, CG3183 gene product from transcript CG3183-RA antibody, geminin antibody, GE24654 gene product from transcript GE24654-RA antibody, GMNN antibody, geminin antibody, gmnn antibody, LOC410093 antibody, Dyak\geminin antibody, Gmnn antibody
    背景
    Geminin inhibits DNA replication by preventing the incorporation of the MCM complex into the prereplication complex (pre-RC). It is absent during the G1 phase, accumulates during the S, G2, and M phases, and disappears at the time of the metaphase-anaphase transition. Geminin contains 212 amino acids and has a destruction box sequence of RRTLKVIQP. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle.Geminin Antibody, pAb, Rabbit is developed in rabbit hosts using a KLH-coupled synthetic peptide within residues 50-100 of human geminin protein (Swiss Prot: O75496).
    途径
    EGFR Signaling Pathway, DNA Replication
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