Rabbit Anti-Calcineurin A Polyclonal Antibody detects endogenous levels of calcineurin A protein. Sequence homology predicts that it will also react with rat calcineurin A.
交叉反应 (详细)
Rabbit Anti-Calcineurin A Polyclonal Antibody detects endogenous levels of calcineurin A protein. Sequence homology predicts that it will also react with rat calcineurin A.
纯化方法
Immunoaffinity chromatography
免疫原
Synthetic peptide (KLH-coupled) within AA 450-500 of human calcineurin A protein
CAN
适用: 人
WB, IP, IHC, ICC, IF
宿主: 兔
Polyclonal
Atto 488
应用备注
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/mL Western blot: 0.5-1 µg/mL Other applications: user-optimized
限制
仅限研究用
状态
Lyophilized
缓冲液
PBS, pH 7.4, containing 0.02 % sodium azide
储存液
Sodium azide
注意事项
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
储存条件
4 °C/-20 °C
储存方法
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Calcineurin is a heterodimer protein that binds to Ca2+. A Ca2+/calmodulin-regulated protein phosphatase, it is composed of a 19 kD calcineurin B (CnB) and a 61 kD calmodulin-binding catalytic subunit, calcineurin A (CnA). Calcineurin A was first detected in skeletal muscle and brain tissues, but it has since been found in all cell types, from yeast to mammalian. Calcineurin A may be responsible for the calmodulin activation property of calcineurin. There is evidence suggesting that alternative splicing could give rise to different forms of calcineurin A.Rabbit Anti-Calcineurin A Polyclonal Antibody is developed in rabbit hosts using a KLH-coupled synthetic peptide within residues 450-500 of human calcineurin A protein (Swiss Prot: Q08209).