Rabbit Anti-Histone 2B Polyclonal Antibody detects endogenous levels of human histone 2B protein. Sequence homology predicts that it will also react with Histone 2B protein within mouse and fruit fly proteins.
交叉反应 (详细)
Rabbit Anti-Histone 2B Polyclonal Antibody detects endogenous levels of human histone 2B protein. Sequence homology predicts that it will also react with Histone 2B protein within mouse and fruit fly proteins.
纯化方法
Immunoaffinity chromatography
免疫原
Synthetic peptide (KLH-coupled) derived from the carboxyl terminal of human histone 2B .
The investigator must determine the working concentrations for each specific application. The appropriate concentration may depend on such factors as secondary antibody affinity, antigen concentration, detection method, temperature, and length of incubation. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/mL Western blot: 0.5-1 µg/mL Other applications: user-optimized
限制
仅限研究用
状态
Lyophilized
缓冲液
PBS, pH 7.4, containing 0.02 % sodium azide
储存液
Sodium azide
注意事项
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
注意事项
Avoid repeated freezing and thawing cycles.
储存条件
4 °C/-20 °C
储存方法
The antibody is stable in lyophilized form if stored at -20 °C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-0 °C. For long term storage, aliquot and store at -20 °C or below.
Histone 2B is the core component of nucleosome. It is involved in nucleosome wrapping and the compaction of DNA into chromatin, limiting DNA accessibility. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated via a complex system of nucleosome remodelling and post-translational modification of histones, also called histone code.Rabbit Anti-Histone 2B Polyclonal Antibody is developed in rabbit hosts using a KLH-coupled synthetic peptide corresponding to carboxyl terminal residues of human histone 2B protein (Swiss Prot: Q99879).