GAPDH 抗体 (Biotin)
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- 抗原 See all GAPDH 抗体
- GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
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适用
- 人, 小鼠, 大鼠, 兔, Pig, 小鸡, Cow, Hamster, 山羊, 鱼
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This GAPDH antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB)
- 特异性
- Goat Anti-GAPDH [Biotin] Polyclonal Antibody specifically reacts with GAPDH from human, mouse, rat, goat, rabbit, chicken, hamster, bovine, swine, fish, and E. coli cell or tissue lysates. It has not yet been tested for other species.
- 交叉反应 (详细)
- Reactivity to other species is not tested yet.
- 亚型
- IgG
- Top Product
- Discover our top product GAPDH Primary Antibody
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- 应用备注
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Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.1-1.0 µg/mL Other applications: user-optimized - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- PBS, pH 7.4, containing 30 % glycerol and 0.02 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
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- 抗原
- GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
- 别名
- GAPDH (GAPDH 产品)
- 别名
- G3PD antibody, GAPD antibody, Gapd antibody, BEST:GH12586 antibody, CG12055 antibody, Dmel\\CG12055 antibody, GA3PDH antibody, GADPH antibody, GAP antibody, GAPDH antibody, GAPDH I antibody, GAPDH-1 antibody, GAPDH1 antibody, GAPDHI antibody, Gapdh antibody, Gapdh-1 antibody, Gapdh43E antibody, gadph antibody, gapdh antibody, gapdh-1 antibody, gh12586 antibody, cb609 antibody, gapd antibody, mg:bb02e05 antibody, wu:fb33a10 antibody, wu:ft80f05 antibody, KNC-NDS6 antibody, g3pd antibody, G3PDH antibody, glyceraldehyde-3-phosphate dehydrogenase antibody, Glyceraldehyde 3 phosphate dehydrogenase 1 antibody, glyceraldehyde-3-phosphate dehydrogenase S homeolog antibody, glyceraldehyde-3-phosphate dehydrogenase, type I antibody, olfactory receptor 8K3 antibody, GAPDH antibody, Gapdh antibody, Gapdh1 antibody, gapdh antibody, gapdh.S antibody, gapDH antibody, LOC100404960 antibody, LOC614985 antibody
- 背景
- Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is one of the key enzymes involved in glycolysis. Though expressed differently in different tissues , GAPDH is thought to be a constitutively expressed housekeeping protein. For this reason, GAPDH is often measured as a control in experiments quantifying specific changes in expression of other targets. Recent evidence suggests that mammalian GAPDH is also involved in a great number of intracellular processes such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. GAPDH may also play a role in neurodegenerative pathologies such as Huntingtons and Alzheimer-disease. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types. Goat Anti-GAPDH [Biotin] Polyclonal Antibody is highly purified from goat antiserum by immunoaffinity chromatography and then conjugated to biotin. The antibody is supplied as a 40 µg aliquot at a concentration of 0.5 mg/ml.
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