(2 references)-
- 抗原 See all Protein C Tag products
- Protein C Tag
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Protein C Tag antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA
- 特异性
- This Antibody recognizes C-terminal, N-terminal, and internal Protein C tagged fusion proteins.
- 纯化方法
- Immunoaffinity chromatography
- 免疫原
- Protein C epitope tag peptide CEDQVDPRLIDGK conjugated - KLH
- 亚型
- IgG
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anti-Protein C Tag antibody
WB, EIA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/mL
Western blot: 0.1-1.0 µg/mLWestern Blot Using ONE-HOUR WesternTM Kit: For quick results, ONE-HOUR WesternTM Complete Kit (Rabbit) is recommended. 10 µg of this antibody is mixed with 10 mL of WB solution for 8 cm X 8 cm membrane.Other applications: user-optimized - 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- PBS, pH 7.4, containing 0.02 %Sodium azide
- 储存液
- Sodium azide
- 注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
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: "Purification of protein C from canine plasma." in: BMC veterinary research, Vol. 10, pp. 251, (2015) (PubMed).
: "Role of EBAG9 protein in coat protein complex I-dependent glycoprotein maturation and secretion processes in tumor cells." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 24, Issue 10, pp. 4000-19, (2010) (PubMed).
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: "Purification of protein C from canine plasma." in: BMC veterinary research, Vol. 10, pp. 251, (2015) (PubMed).
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- 抗原
- Protein C Tag
- Abstract
- Protein C Tag 产品
- 物质类
- Tag
- 背景
- Rabbit Anti-Protein C (HPC4)-tag is highly purified from rabbit antiserum by immunoaffinity chromatography and is supplied as a 40 µg aliquot at a concentration of 1 mg/ml in PBS, pH7.4, containing 0.02% Sodium azide.
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