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GFP 抗体

Name: GFP 抗体
Brand: antibodies-online
SKU: ABIN1573884
Availability: OutOfStock

GFP 适用: Aequorea victoria WB, ELISA 宿主: 小鼠 Monoclonal 5E5G11 unconjugated

Lane 1-3: 200 ng, 40 ng, 8 ng GFP fusion proteinDetection antibody: Mouse Anti-cGFP-tag Monoclonal Antibody (ABIN398417) The Western blot was performed using One-Step WesternTM Basic Kit (ABIN491503) with 4 µg of the antibody added to 4 mL WB solution. (GFP 抗体)

Lane 1-3: 100 ng, 25 ng, 10 ng GFP fusion proteinLane 4: Negative controlPrimary antibody: A. (GFP 抗体)

Lane 1: OFP transfecting 293 cell lysateLane 2: EGFP transfecting 293 cell lysateLane 3: 5 ng GFPuv proteinLane 4: Negative 293 cell lysate Primary antibody: A. (GFP 抗体)

3 images

(6 references)

产品编号 ABIN1573884

发货至: 中国

Datasheet as PDF

抗原 See all GFP 抗体

GFP (Green Fluorescent Protein (GFP))
适用
176

15

11

9
Aequorea victoria
宿主
64

62

35

15

9

5

1

1
小鼠
Compatible Secondaries
克隆类型
115

72
单克隆
标记
82

13

11

10

5

4

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This GFP antibody is un-conjugated
应用范围
150

76

46

33

25

25

25

21

14

13

13

12

12

5

4

4

2

2

1

1

1

1

1
Western Blotting (WB), ELISA

特异性

The antibody reacts with various forms of GFP including eGFP, OFP, and GFPuv.

纯化方法

Protein G affinity column

免疫原

Purified recombinant cGFP fusion protein

克隆位点

5E5G11

亚型

IgG

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GFP 适用: Aequorea victoria WB, IF, IHC (fro) 宿主: 山羊 Polyclonal DyLight 550

应用备注

The investigator must determine the ideal working concentration for each specific application. We have not yet determined the suitability of this antibody for applications other than those listed below. The ideal working concentration must take into account such factors as secondary antibody affinity, antigen concentration, sensitivity of the detection method, temperature, and length of incubationsELISA: 0.1-1.0 µg/mL
Western blot: 1.0-2.0 µg/mLWestern blot using ONE-HOUR WesternTM Kit: For quick results, ONE-HOUR WesternTM Kit is recommendet. 4 µg of this antibody is mixed with 4 mL of WB solution.
Other applications: user-optimized

限制

仅限研究用
状态

Lyophilized

缓冲液

PBS, pH 7.4, containing 0.02 % Sodium azide

储存液

Sodium azide

注意事项

WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

储存条件

4 °C/-20 °C

储存方法

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Li, Shen, Sun, Wang, Li, Huang, Chen, Li, Zhao, Luo, Li, Qiu: "Effects of the nuclear localization of the N(pro) protein of classical swine fever virus on its virulence in pigs." in: Veterinary microbiology, Vol. 174, Issue 3-4, pp. 391-8, (2014) (PubMed).

Liu, Yu, Wu, Cao, Wu, Wu, Wang: "Characterization of the open reading frame 7a from Bombyx mori nucleopolyhedrovirus." in: Molecular biology reports, Vol. 40, Issue 2, pp. 865-73, (2013) (PubMed).

Liu, Zhu, Wu, He, Chen: "Inhibition effect produced by dominant negative mutant fusion protein PreS2-TLM-ScFv-HBcDN on HBV replication in vitro." in: Journal of viral hepatitis, Vol. 19, Issue 4, pp. 295-300, (2012) (PubMed).

Wilusz, JnBaptiste, Lu, Kuhn, Joshua-Tor, Sharp: "A triple helix stabilizes the 3' ends of long noncoding RNAs that lack poly(A) tails." in: Genes & development, Vol. 26, Issue 21, pp. 2392-407, (2012) (PubMed).

Yang, Jin, Chen, Lin, Wang, Chen, Han, Bian, Zhu, Wang: "A chloroplast envelope membrane protein containing a putative LrgB domain related to the control of bacterial death and lysis is required for chloroplast development in Arabidopsis thaliana." in: The New phytologist, Vol. 193, Issue 1, pp. 81-95, (2011) (PubMed).

Qi, Wang, Shen, Zhang, Chen, Xu, Liu, Wu, Jiang: "OsARF12, a transcription activator on auxin response gene, regulates root elongation and affects iron accumulation in rice (Oryza sativa)." in: The New phytologist, Vol. 193, Issue 1, pp. 109-20, (2011) (PubMed).
抗原

GFP (Green Fluorescent Protein (GFP))

别名

cGFP (GFP 产品)

别名

green fluorescent protein antibody, gfp antibody

背景

The coral green fluorescent protein (cGFP) from vector pRNATin-H1.2/Neo (Antibodies-Online, SD1223) is a versatile marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression. Mouse Anti-cGFP-tag Monoclonal Antibody can be used to detect cGFP fusion protein in Western blot analyses.