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Myc Tag 抗体

Name: Myc Tag 抗体
Brand: antibodies-online
SKU: ABIN1573879
Availability: OutOfStock

WB, IP, ELISA, IF 宿主: 兔 Polyclonal unconjugated

Western blot analysis of c-Myc fusion protein (MW~52KD) using 1 µg/mL Rabbit Anti-c-Myc-tag Polyclonal Antibody (ABIN398404) Lane 1-3: c-Myc fusion protein in HEK293 cell lysateLane 4: Negative HEK293 cell lysateSecondary antibody: Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (ABIN398323, 1: 20,000) The signal was developed with LumiSensorTM HRP Substrate Kit (ABIN769939) (Myc Tag 抗体)

Immunofluorescent analysis of human kidney tissue slide usingRabbit Anti-c-Myc-tag Polyclonal Antibody (ABIN398404) (Myc Tag 抗体)

Western blot analysis of c-Myc tagged fusion proteins expressed in E. (Myc Tag 抗体)

3 images

(12 references)

产品编号 ABIN1573879

发货至: 中国

Datasheet as PDF

抗原 See all Myc Tag products

Myc Tag

适用

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宿主
74

49

6

5

5

5

1

1
兔
Compatible Secondaries
克隆类型
76

58

10
多克隆
标记
66

13

10

7

3

3

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This Myc Tag antibody is un-conjugated
应用范围
109

53

42

36

25

23

20

18

15

11

11

6

6

5

4

4

4

3

2

2

1

1

1

1

1

1
Western Blotting (WB), Immunoprecipitation (IP), ELISA, Immunofluorescence (IF)

序列

EQKLISEEDL

特异性

This Antibody recognizes C-terminal, N-terminal, and internal c-Myc tagged fusion proteins.

纯化方法

Immunoaffinity chromatography

免疫原

c-Myc epitope tag peptide EQKLISEEDL conjugated - KLH

亚型

IgG
anti-Myc Tag antibody
WB, IP, ELISA, IF 宿主: 小鼠 Monoclonal unconjugated

anti-Myc Tag (C-Term) antibody
适用: 人 WB, IP, IHC (p), FACS 宿主: 小鼠 Monoclonal 9E10 unconjugated

anti-Myc Tag antibody
WB, IP, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Myc Tag (C-Term) antibody (FITC)
适用: 人 FACS 宿主: 小鼠 Monoclonal 9E10 FITC

anti-Myc Tag (C-Term) antibody (Biotin)
适用: 人 WB, IP, IHC (p), FACS 宿主: 小鼠 Monoclonal 9E10 Biotin

anti-Myc Tag antibody
WB, IP, ELISA, IF 宿主: 小鼠 Monoclonal 2G8D5 unconjugated

anti-Myc Tag (AA 408-439) antibody
WB, ELISA 宿主: 小鼠 Monoclonal 9E10- unconjugated

anti-Myc Tag antibody
WB, IP, IF 宿主: 小鼠 Monoclonal 3B8 unconjugated

anti-Myc Tag (AA 410-419) antibody
WB 宿主: 山羊 Polyclonal unconjugated

anti-Myc Tag (AA 408-439) antibody
适用: 人 WB, IP, IF, IHC (fro), IHC (p) 宿主: 小鼠 Monoclonal 9E10 unconjugated Recombinant Antibody

应用备注

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL
Western blot: 0.1-1.0 µg/mLWestern blot using ONE-HOUR WesternTM Kit: For quick results, ONE-HOUR WesternTM Kit is recommendet. 10 µg of this antibody is mixed with 10 mL of WB solution for 8 cm x 8 cm membrane.Immunofluorescence: 5-20 µg/mLImmunoprecipitation (IP): 2-5 µg/mL
Other applications: user-optimized

限制

仅限研究用
状态

Lyophilized

溶解方式

Reconstitute the lyophilized product with deionized water (or equivalent) to the final concentration of 0.5 mg/ml.

缓冲液

PBS, pH 7.4, containing 0.02 % Sodium azide

储存液

Sodium azide

注意事项

WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

储存条件

4 °C/-20 °C

储存方法

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Capella, Ré, Arce, Chan: "Plant homeodomain-leucine zipper I transcription factors exhibit different functional AHA motifs that selectively interact with TBP or/and TFIIB." in: Plant cell reports, Vol. 33, Issue 6, pp. 955-67, (2014) (PubMed).

Armengot, Marquès-Bueno, Soria-Garcia, Müller, Munné-Bosch, Martínez: "Functional interplay between protein kinase CK2 and salicylic acid sustains PIN transcriptional expression and root development." in: The Plant journal : for cell and molecular biology, Vol. 78, Issue 3, pp. 411-23, (2014) (PubMed).

Berim, Park, Gang: "Unexpected roles for ancient proteins: flavone 8-hydroxylase in sweet basil trichomes is a Rieske-type, PAO-family oxygenase." in: The Plant journal : for cell and molecular biology, Vol. 80, Issue 3, pp. 385-95, (2014) (PubMed).

Chen, Sheng, Liu, Yao, Gao, Song, Jiang, Li, Huang: "Enhancer of zeste homolog 2 is a negative regulator of mitochondria-mediated innate immune responses." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 191, Issue 5, pp. 2614-23, (2013) (PubMed).

Zhang, Xiang, Zhong, Yin, Gu, Deng: "The p16-specific reactivation and inhibition of cell migration through demethylation of CpG islands by engineered transcription factors." in: Human gene therapy, Vol. 23, Issue 10, pp. 1071-81, (2012) (PubMed).

Li, Che, Zou, Cui, Guo, Zou, Biddle, Yang, Chen: "Hpa2 required by HrpF to translocate Xanthomonas oryzae transcriptional activator-like effectors into rice for pathogenicity." in: Applied and environmental microbiology, Vol. 77, Issue 11, pp. 3809-18, (2011) (PubMed).

Xu, Zhang, Schläppi, Xu: "Cold-inducible expression of AZI1 and its function in improvement of freezing tolerance of Arabidopsis thaliana and Saccharomyces cerevisiae." in: Journal of plant physiology, Vol. 168, Issue 13, pp. 1576-87, (2011) (PubMed).

Masuho, Wakasugi-Masuho, Posokhova, Patton, Martemyanov: "Type 5 G protein beta subunit (Gbeta5) controls the interaction of regulator of G protein signaling 9 (RGS9) with membrane anchors." in: The Journal of biological chemistry, Vol. 286, Issue 24, pp. 21806-13, (2011) (PubMed).

Georgakopoulos, Xu, Xu, Mauger, Barthet, Robakis: "Presenilin1/gamma-secretase promotes the EphB2-induced phosphorylation of ephrinB2 by regulating phosphoprotein associated with glycosphingolipid-enriched microdomains/Csk binding protein." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 25, Issue 10, pp. 3594-604, (2011) (PubMed).

Zheng, Li, Zhang, Meng, Mao, Bai, Lu, Mao, Hu, Wang: "Enterovirus 71 2C protein inhibits TNF-?-mediated activation of NF-?B by suppressing I?B kinase ? phosphorylation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 187, Issue 5, pp. 2202-12, (2011) (PubMed).

Lo, Chen: "CCAAT/enhancer binding protein beta2 is involved in growth hormone-regulated insulin-like growth factor-II gene expression in the liver of rainbow trout (Oncorhynchus mykiss)." in: Endocrinology, Vol. 151, Issue 5, pp. 2128-39, (2010) (PubMed).

Li, Yue, Deng, Ueda, Fukunaga, Khuri, Sun: "Protein phosphatase 2A negatively regulates eukaryotic initiation factor 4E phosphorylation and eIF4F assembly through direct dephosphorylation of Mnk and eIF4E." in: Neoplasia (New York, N.Y.), Vol. 12, Issue 10, pp. 848-55, (2010) (PubMed).
抗原

Myc Tag

别名

C-Myc-Tag (Myc Tag 产品)

物质类

Tag

背景

Rabbit Anti-c-Myc-tag Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and is supplied as a 40 µg aliquot at a concentration of 1 mg/ml in PBS, pH 7.4, containing 0.02% Sodium azide.