This affinity-purified antibody is directed against Human MAGP-2 protein. A BLAST analysis was used to suggest cross reactivity with MAGP-2 protein from Human and chimpanzee based on 100 % homology with the immunizing sequence. Expect partial reactivity with MAGP-2 from Mouse, Dog and Rat sources based on partial (~75 %) sequence homology. Reactivity against homologues from other sources is not known.
交叉反应 (详细)
Species reactivity (tested):Human, Chimpanzee.
纯化方法
Immunoaffinity (from monospecific antiserum).
免疫原
Synthetic peptide corresponding to a region near the carboxy terminal end of human MAGP-2 protein. Remarks: Full length protein sequence for human MAGP-2 (173 aa, predicted MW 19.6 kDa): 1 msllgpkvll flaafiitsd wiplgvnsqr gddvtqatpe tftedpnlvn dpatdetvla 61 vladiapstd dlaslseknt taecwdekft ctrlysvhrp vkqcihqlcf tslrrmyivn 121 keicsrlvck eheamkdelc rqmaglpprr lrrsnyfrlp pcenvdlqrp ngl
This affinity-purified antibody has been tested for use in ELISA (1/5,000-1/20,000) andWestern blot (1/250-1/1,000). Specific conditions for reactivity should be optimized by theend user. Expect a band approximately 20-30 kDa in size corresponding to MAGP-2 proteinby Western blotting in the appropriate cell lysate or extract. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
0.95 mg/mL (by UV absorbance at 280 nm)
缓冲液
0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing. Dilute only prior to immediate use
储存条件
4 °C/-20 °C
储存方法
Store vial at 2-8 °C prior to restoration. Restore with deionized water r equivalent, this antibody is stable for one month at 2-8 °C as an undiluted liquid. For extended storage aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature.
抗原
MFAP5
(Microfibrillar Associated Protein 5 (MFAP5))
MAGP-2 is a secreted, extracellular matrix protein which is a component of the elastin-associated microfibrils. It forms intermolecular disulfide bonds either with other MAGP-2 Molecules or with other components of the microfibrils. MAGP-2 is upregulated in some ovarian cancers and is associated with poor survival of ovarian cancer patients.Synonyms: MAGP2, MFAP-5, MP25, Microfibril-associated glycoprotein 2, Microfibrillar-associated protein 5