ELISA: Use at 1-15 μg/mL. Western blot: Use at 1-2 μg/mL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
溶解方式
Restore in sterile water to a concentration of 1.0 mg/mL.
缓冲液
PBS, pH 7.4 without preservative or stabilizer.
储存液
Without preservative
储存条件
4 °C
储存方法
The lyophilized antibody, thought stable at room temperature, is best stored desiccated below 0 °C. Reconstituted anti-VEGF-A is stable at 4 °C for six weeks or can be stored in working aliquots at - 20 °C for longer.
抗原
VEGFA
(Vascular Endothelial Growth Factor A (VEGFA))
VEGFA is a member of the PDGF/VEGF growth factor family and is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternate transcriptional splice variants, encoding either freely secreted or cell associated isoforms, have been characterized. There is also evidence for the use of non AUG (CUG) translation initiation sites upstream of, and in frame with the first AUG, leading to additional isoforms.Synonyms: VEGF, VEGFA, VPF, Vascular endothelial growth factor A, Vascular permeability factor