Podoplanin 抗体
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- 抗原 See all Podoplanin (PDPN) 抗体
- Podoplanin (PDPN)
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适用
- 小鼠
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宿主
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仓鼠
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克隆类型
- 单克隆
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标记
- This Podoplanin antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 特异性
- The monoclonal antibody will detect Podoplanin on the surface of lymphatic endothelial cells and some epithelial cell types by immunostaining or immunohistochemistry. The antibody does not cross-react with Human Podoplanin.
- 纯化方法
- Protein G Chromatography
- 免疫原
- Murine thymic stromal cell line
- 克隆位点
- RTD4E10
- 亚型
- IgG
- Top Product
- Discover our top product PDPN Primary Antibody
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- 应用备注
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Immunohistochemistry (use diluted 1-10 μg/mL IgG). Works well with paraffin sections. Western blot (use 1-10 μg/mL IgG, will detect a 36-40 kDa band). Immunoprecipitation (use 1-5 μg IgG per 1 mLlysate or reaction volume).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 溶解方式
- Restore with sterile water to a concentration of 0.1-1.0 mg/mL.
- 缓冲液
- PBS, pH 7.4, without preservatives or stabilizers
- 储存液
- Without preservative
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Prior to reconstitution store at 2-8 °C. After reconstitution store the antibody (in aliquots) at -20 °C.
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Novel function for blood platelets and podoplanin in developmental separation of blood and lymphatic circulation." in: Blood, Vol. 115, Issue 19, pp. 3997-4005, (2010) (PubMed).
: "Regulation of lymphatic-blood vessel separation by endothelial Rac1." in: Development (Cambridge, England), Vol. 136, Issue 23, pp. 4043-53, (2009) (PubMed).
: "Regulation of erythropoiesis. I. Erythropoietin assay as a clinical tool." in: Blood, Vol. 20, pp. 9-18 (PubMed).
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Novel function for blood platelets and podoplanin in developmental separation of blood and lymphatic circulation." in: Blood, Vol. 115, Issue 19, pp. 3997-4005, (2010) (PubMed).
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- 抗原
- Podoplanin (PDPN)
- 别名
- Podoplanin (PDPN 产品)
- 别名
- PDPN antibody, AGGRUS antibody, GP36 antibody, GP40 antibody, Gp38 antibody, HT1A-1 antibody, OTS8 antibody, PA2.26 antibody, T1A antibody, T1A-2 antibody, OTS-8 antibody, RANDAM-2 antibody, T1-alpha antibody, T1a antibody, T1alpha antibody, E11 antibody, RTI40 antibody, podoplanin antibody, PDPN antibody, Pdpn antibody
- 背景
- Podoplanin is a well-recognized lymphatic endothelium marker, which can be used to reliably distinguish lymphatic vessels from blood vessels. Podoplanin is a Mr 38,000 membrane mucoprotein that was originally detected on the surface of rat glomerular epithelial cells (podocytes) and was found to be linked to flattening of foot processes that occurs in glomerular diseases. Podoplanin shows features of a membrane mucoprotein with several conserved O-glycosylation sites. Currently, it is of unknown biological function. Because heavily O-glycosylated mucoproteins were identified recently as counterreceptors for selectins that mediate adhesion of inflammatory cells, it is possible that podoplanin plays a similar role in lymphatic endothelia.Synonyms: Aggrus, GP36, Glycoprotein 36, PA2.26 antigen, PDPN, PSEC0003, PSEC0025, T1-alpha
- 基因ID
- 14726
- NCBI登录号
- NP_034459
- UniProt
- Q62011
- 途径
- Dicarboxylic Acid Transport
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