FACS analysis and cell sorting: 2-5 μg/mL. ELISA. Western Blot. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
溶解方式
Restore to a concentration of 50 μg/mL with sterile PBS solution containing 0.1 % BSA.
缓冲液
0.1 M Tris-Cl, 0.1 M NaCl, pH 7.4, 0.02 % Sodium Azide, 50x BSA
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
This product is photosensitive and should be protected from light
储存条件
4 °C
储存方法
Prior to and following reconstitution store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
TIE2 (tyrosine kinase with Ig and EGF homology domains 2) is expressed almost exclusively in endothelial cells in mice, rats and humans. This receptor possesses a unique extracellular domain containing two immunoglobulin like loops separated by three epidermal growth factor like repeats that are connected to three fibronectin type III like repeats. The ligand for the receptor is Angiopoietin 1. Defects in TIE2 are associated with inherited venous malformations, the TIE2 signaling pathway appears to be critical for endothelial cell smooth muscle cell communication in venous morphogenesis.Synonyms: Angiopoietin-1 receptor, TIE-2, TIE2, Tunica interna endothelial cell kinase, Tyrosine-protein kinase receptor TEK, Tyrosine-protein kinase receptor TIE-2, p140 TEK