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PKC alpha 抗体 (pThr638)

Name: PKC alpha 抗体 (pThr638)
SKU: ABIN1531462
Availability: OutOfStock

PKCa 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

Western blot analysis of extracts from NIH-3T3 cells treated with UV 15', using PKC alpha (Phospho-Thr638) Antibody. (PKC alpha 抗体 (pThr638))

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using PKC alpha (Phospho-Thr638) Antibody. (PKC alpha 抗体 (pThr638))

IHC

2 images

(1 reference)

产品编号 ABIN1531462

发货至: 中国

Datasheet as PDF

抗原 See all PKC alpha (PKCa) 抗体

PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))
抗原表位
23

20

16

12

10

9

8

7

7

6

5

4

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 606-655, pThr638
适用
161

88

65

5

5

3

3

2

2

1

1

1

1
人, 小鼠, 大鼠
宿主
159

10

1
兔
Compatible Secondaries
克隆类型
140

29
多克隆
标记
81

14

10

6

6

6

6

6

6

6

4

4

2

2

1

1

1

1

1

1

1

1

1

1

1
This PKC alpha antibody is un-conjugated
应用范围
113

65

52

22

18

16

16

15

14

7

5

2

1

1

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

特异性

PKC alpha (Phospho-Thr638) Antibody detects endogenous levels of PKC alpha only when phosphorylated at Thr638.
PhosphorylationH:T638 M:T638 R:T638

纯化方法

The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

纯度

> 95 %

免疫原

The antiserum was produced against synthesized peptide derived from human PKC alpha around the phosphorylation site of Thr638.

亚型

IgG

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anti-Protein Kinase C, alpha (PKCa) antibody
PKCa 适用: 人, 小鼠, 大鼠 WB, IHC, IP, FACS, IF, ICC 宿主: 兔 Monoclonal unconjugated

anti-Protein Kinase C, alpha (PKCa) (AA 299-329) antibody
PKCa 适用: 人 WB, ELISA, IHC, FACS, IF 宿主: 兔 Polyclonal unconjugated

anti-Protein Kinase C, alpha (PKCa) (pThr638) antibody
PKCa 适用: 人, 小鼠 WB, FACS, IHC (p), IF (cc) 宿主: 兔 Monoclonal 4B3 unconjugated

anti-Protein Kinase C, alpha (PKCa) (pThr638) antibody
PKCa 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Protein Kinase C, alpha (PKCa) antibody
PKCa 适用: 人, 小鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Protein Kinase C, alpha (PKCa) (AA 299-329), (C-Term) antibody
PKCa 适用: 人 WB, FACS, IF, IHC (p) 宿主: 兔 Polyclonal RB01012 unconjugated

anti-Protein Kinase C, alpha (PKCa) (C-Term) antibody
PKCa 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Protein Kinase C, alpha (PKCa) (N-Term) antibody
PKCa 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Protein Kinase C, alpha (PKCa) antibody
PKCa 适用: 人 WB, IHC, IP 宿主: 兔 Monoclonal unconjugated

应用备注

WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

说明

Unigene-Number: Hs.531704, Hs.708867 (NCBI Gene Symbol: PRKCA)

限制

仅限研究用
状态

Liquid

浓度

1 mg/mL

缓冲液

phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Stable at -20°C for at least 1 year.

有效期

12 months
Huang, Cheng, Su, Lin: "Syndecan-4 Promotes Epithelial Tumor Cells Spreading and Regulates the Turnover of PKC? Activity under Mechanical Stimulation on the Elastomeric Substrates." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 36, Issue 4, pp. 1291-1304, (2015) (PubMed).
抗原

PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

别名

PKC alpha (PKCa 产品)

别名

pkc-alpha antibody, pkcalpha antibody, im:7139045 antibody, si:dkey-8l19.1 antibody, LOC100219195 antibody, AAG6 antibody, PKC-alpha antibody, PKCA antibody, PRKACA antibody, AI875142 antibody, Pkca antibody, PKRCA antibody, protein kinase C alpha antibody, protein kinase C, alpha L homeolog antibody, protein kinase C, alpha antibody, PRKCA antibody, prkca.L antibody, prkca antibody, Prkca antibody

背景

Synonyms: kinase PKC-alpha, KPCA, PKC III, PKC-A, PKC-alpha, PKC-III, PRKACA, PRKCA, Protein kinase C, alpha type
NCBI Gene Symbol: PRKCA

分子量

76 kDa

基因ID

5578

OMIM

176960

UniProt

P17252

途径

WNT signaling, TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling